3^rd World Congress on Pharmacology August 08-10, 2016 Birmingham, UK

Biography:

Chia-Wen Tsai received her PhD degree (2006) in Department of Nutrition from Chung Shan Medical University. She has been working as a Associate Professor at the Department of Nutrition, China Medical University in Taiwan. Her research interests include the herbs in prevention of neurodegenerative diseases, the modulations of glucose and lipid metabolism in herbs, and the functional foods in cancer chemoprevention.

Abstract:

Understanding the neuroprotective effects of carnosic acid (CA) from rosemary has attracted increasing attention in recent years. Parkin promotes the degradation of mitochondria pro-apoptotic ARTS (apoptosis-related protein in the TGF-β signaling pathway) protein and prevents it antagonizes XIAP (X-linked inhibitors of apoptosis proteins) leading to caspase activation. In this present study, we explored whether CA could prevent 6-hydroxydopamine (6-OHDA)-induced apoptosis via modulation of ARTS  and XIAP by parkin in SH-SY5Y cells. The results showed that pretreatment cells with CA reduced 6-OHDA-induced the nuclear condensation and apoptotic proteins. Treatment cells with 6-OHDA increased ARTS protein and decreased XIAP protein as well as parkin protein. However, CA pretreatment reversed the effect by 6-OHDA. Performing immunoprecipitation using an anti-ARTS antibody, 6-OHDA increased the XIAP protein. In contrast, CA pretreatment reduced XIAP protein and increased ubiquitin protein in 6-OHDA treated cells. Silencing of XIAP reduced the CA inhibited 6-OHDA-increased the cleavage of caspase 9, 3, 7 and PARP. With parkin siRNA, CA reversed the 6-OHDA-induced ARTS and reduced XIAP was inhibited, which resulted in increasing apoptotic related proteins. In conclusion, CA protects SH-SY5Y cells against 6-OHDA-stimulated apoptosis via induction of parkin by enhancing the ubiquitination of ARTS, leading to induction of XIAP.

Biography:

Syed Arman Rabbani has completed his PhD in Clinical Pharmacology from the Clinical Pharmacology Unit, Ranbaxy Research Laboratories in collaboration with Jamia Hamdard University, New Delhi, India. He is a University Gold Medalist. Currently, he is working as an Assistant Professor in Department of Clinical Pharmacy and Pharmacology, RAK College of Pharmaceutical Sciences, RAK Medical and Health Sciences University. He has a rich blend of experience in both industry as well as academics. In industry, he was involved in the conceptualization and conduct of Phase III and Phase IV Clinical Trials.

Abstract:

The objective of the study was to assessthe prevalence of hyperphosphatemia in End Stage Renal Disease patients (ESRD) undergoing maintenance hemodialysis at a tertiary care hospital in Ras Al Khaimah, UAE. The study also aimed to describe the characteristics of the ESRD patients with hyperphosphatemia. The study was a prospective, observational study including all the patients undergoing hemodialysis at the nephrology unit of the hospital. Patients who were on hemodialysis for less than six months, with less than thrice weekly hemodialysis and with acute kidney injury were excluded from the study. Patient’s characteristics were compared according to phosphatemia level, between patients with or without hyperphosphatemia of the 80 patients included (mean age, 61.1 ± 9.4 years), 73.8% had hyperphosphatemia, defined by a mean serum phosphate > 1.45 mmol/L. Patients with hyperphosphatemia are younger and have a higher BMI than those with normal phosphate levels, and more often women. They were more likely to have hypertensive and diabetic nephropathy, and had less comorbidity. The study showed that ESRD patients with hyperphosphatemia differ from those with normal phosphate levels.

Biography:

Lihua Sun, Associate Professor of Pharmacology, has completed her PhD at the age of 29 years from Harbin Medical University and postdoctoral studies from Harbin Medical University. She is working on the research and teaching of Pharmacology, the research interest focuses on Cardio-Cerebral Vascular pharmacology. She has published more than 35 papers in international peer-reviewed journals and has been supported by series of national scientific foundations and has been serving as a Reviewer for PLOS ONE and Gene. She obtained the rewards from Ministry of Education of China due to her success in researches.

Abstract:

Previous studies have demonstrated that the trafficking defects of Nav1.1/Nav1.2 are involved in the dementia pathophysiology. However, the detailed mechanisms are not fully understood. Moreover, whether the impaired miRNAs regulation linked to dementia is a key player in sodium channel trafficking disturbance remains unclear. The cognitive impairment induced by chronic cerebral ischemia through chronic brain hypoperfusion (CBH) by bilateral common carotid artery occlusion (2VO) is likely reason to precede dementia. Our results found the impairment of Nav1.1/Nav1.2 trafficking and decreased expression of Navβ2 and increased miR-9 in the hippocampus and cortex of rats following CBH generated by bilateral 2VO. Intriguingly, MiR-9 suppressed, while AMO-miR-9 enhanced, the trafficking of Nav1.1/Nav1.2 from cytoplasm to cell membrane. Further study showed that overexpression of miR-9 inhibited the expression of Navβ2 by targeting on its coding sequence (CDS) domain by dual luciferase assay. However, binding-site mutation or miR-masks failed to influence Navβ2 expression as well as Nav1.1/Nav1.2 trafficking process, indicating that Navβ2 is a potential target for miR-9. Lentivirus-mediated miR-9 overexpression also inhibited Navβ2 expression and elicited translocation deficits to cell membrane of Nav1.1/Nav1.2 in rats, whereas injection of lentivirus-mediated miR-9 knockdown could reverse the impaired trafficking of Nav1.1/Nav1.2 triggered by 2VO. We conclude that miR-9 may play a key role in regulating the process of Nav1.1/Nav1.2 trafficking via targeting on Navβ2 protein in 2VO rats at post-transcriptional level, and inhibition of miR-9 level may be a potentially valuable approach to prevent Nav1.1/Nav1.2 trafficking disturbance induced by CBH.

Biography:

Jing Ai has completed her PhD from Department of Pharmacology, Harbin Medical University and Post-doctoral studies from College of Bioinformatics Science and Technology and Bio-pharmaceutical Key Laboratory of Heilongjiang Province, Harbin Medical University. She has published more than 40 papers in reputed journals and has been serving as an Editorial Board Member of PloS One.

Abstract:

Large cohort studies have revealed a close relationship between cognitive impairment and cardiovascular diseases, although the mechanism underlying this relationship remains incompletely understood. In this study, using a transgenic (Tg) mouse model of cardiac-specific over-expression of microRNA-1-2 (miR-1-2), we observed that microRNA-1 (miR-1) levels were increased not only in the heart but also in the hippocampus and blood, whereas its levels did not change in the skeletal muscle of Tg mice compared with age-matched wild type (WT) mice. Six-month-old Tg mice showed cognitive impairment compared with age-matched WT mice, as assessed using the Morris Water Maze test. The brain-derived neurotrophic factor (BDNF) level and cyclic AMP-responsive element-binding protein (CREB) phosphorylation were also significantly reduced in the hippocampi of the Tg mice, as evaluated by western blot. Further examination showed that BDNF proteins expression was down- or up-regulated by miR-1 over-expression or inhibition, respectively, and was unchanged by binding site mutations or miRNA-masks for the 3’UTR of Bdnf, indicating that this gene is a potential target of miR-1. Knockdown of miR-1 by hippocampal stereotaxic injection of an anti-miR-1 oligonucleotide fragment carried by a lentivirus vector (lenti-pre-AMO-miR-1) led to up-regulation of BDNF expression and prevented the reduction in cognitive performance in the Tg mice without affecting cardiac function. Our findings demonstrate that cardiac over-expression of miR-1 also induces behavioral abnormalities that may be associated, at least in part, with the down-regulation of BDNF expression in the hippocampus. This study definitely contributes to the understanding of the relationship between cardiovascular disease and cognitive impairment.

Biography:

Cherine A Ismail has completed her PhD at the age of 26 years from Alexandria University and postdoctoral studies from Alexandria University School of Medicine. She is a lecturer in Clinical Pharmacology Department, Alexandria University School of Medicine. She has published 8 papers in reputed journals.

Abstract:

Sporadic dementia of Alzheimer’s type is a progressive neurodegenerative disorder. While, Alzheimer’s disease (AD) patients have an increased risk of developing seizures, epileptic activity triggers a variety of inhibitory compensatory responses in hippocampal circuits to counteract imbalances in network activity, interfering with normal neuronal/synaptic functions required for learning and memory. Since, antiepileptic drugs (AEDs) have been claimed to variably modulate cognition, a concern is raised about the most favorable AEDs member in such indication. The study compared the impact of relatively newer AEDs (gabapentin/levetiracetam) versus an old one (carbamazepine) on cognitive impairment-induced by intracerebroventricular (icv) colchicine injection in rats. Fifty-six male Wistar rats were equally assigned into control (normal, aCSF-injected, and vehicle-treated AD) and treated groups. Following icv colchicine injection (15μg/5μl/bilaterally), rats (8-rat/group) randomly received gabapentin (60mg/kg/day), levetiracetam (100mg/kg/day), carbamazepine (100mg/kg/day), donepezil (2mg/kg/day), or vehicle orally once daily for 26 days. Behavioral assessment was done using Morris water maze (days 13, 14 and 21 post-surgery), and passive avoidance (days 25/26) tasks. Biochemically, oxidative stress (malondialdehyde and reduced-glutathione), cholinergic deficit (acetylcholinesterase (AChE) activity), and neurotrophic (Brain-derived neurotrophic factor (BDNF)) parameters were measured in hippocampus and prefrontal cortex. Gabapentin, and levetiracetam significantly improved colchicine-induced cognitive impairment, oxidative stress, and reduced BDNF level. Gabapentin increased AChE activity, while levetiracetam decreased it, in both tissues. Despite the increase in BDNF, carbamazepine didn’t significantly improve colchicine-induced cognitive impairment, oxidative stress, or cholinergic deficit. New AEDs, particularly levetiracetam, improved cognitive function and obviously protected both hippocampus and prefrontal cortex from colchicine deleterious effects. Based on its significant neuroprotection, levetiracetam might be the most favorable AEDs to be used in Alzheimer’s-like dementia.

Biography:

Xin Liang received her PhD degree in Biochemical Engineering from East China University of Science and Technology in 2012, studying antineoplastic agents and pharmacology. Did her postdoctoral research in Renhao Li Lab in Department of Pediatrics, Emory University, USA in 2015; she became a lecturer in School of Pharmacy, East China University of Science and Technology.

Abstract:

With the development of colorectal tumor, it gradually formed the hypoxic tumor microenvironment, and infiltration of a large number of inflammatory cells. The mast cell is a kind of immune cells which can secrete a variety of effector molecules, and plays an important role in the angiogenesis of tumor. But whether MC in colorectal cancer is to promote tumor growth or inhibition of tumor growth is not clear, the role of mast cell-derived HIF-1α in the regulation of mast cell function itself in the lack source of oxygen conditions remains to be elucidated. Here, we analysis of the clinical samples of colorectal cancer, immunohistochemical detection observed that carcinoma tissues and tissues adjacent to colorectal carcinoma were infiltrated with large numbers of mast cells, and the mast cell infiltration quantity increased with the Duke’s stage. The survival time of mast cell deficient mice which bearing colorectal carcinoma was remarkably longer than wild type C57BL/6 mice bearing colorectal carcinoma, the growth of tumor was the same. And this weakens recovered in mast cell reconstruction mice. Further in vitro experiments show that when inhibit the expression of HIF-1α through HIF-1α siRNA in mast cells, the release of inflammatory factors in mast cells reduced and also the degranulation degree of mast cells. This suggested that mast-cell derived hypoxia-inducible 1α(HIF-1α) play an important role in regulating the function of mast cells. According to phenomenon observed, we think that the mast cells promote the development of colorectal cancer, and mast-cell derived hypoxia-inducible 1α (HIF-1α) play an important role in regulating the function of mast cells. Therefore, our study revealed a novel role for mast cell-derived HIF-1α in the colorectal carcinoma microenvironment and has important implications for the design of therapeutic strategies.

Biography:

Ke Xu received his PhD degree in Medicinal Chemistry from East China University of Science and Technology in 2013. After that, he worked in Winship cancer center of Emory University as a post-doctor fellow in the United State. At June 2015, he became a Research Associate of Putuo Hospital and Cancer Institute, Shanghai University of Traditional Chinese Medicine, study on pharmacology of traditional Chinese Medicine and the mechanism of miRNA in the gastrointestinal carcinoma.

Abstract:

Colorectal carcinoma is a frequent cause of cancer-related death in men and women. miRNAs (microRNAs) are endogenous small non-coding RNAs that regulate gene expression negatively at the post-transcriptional level. In the present study we investigated the possible role of microRNAs in the development of MDR (multidrug resistance) in colorectal carcinoma cells. We analysed miRNA expression levels between MDR colorectal carcinoma cell line HCT116/L-OHP cells and their parent cell line HCT116 using a miRNA microarray. miR-297 showed lower expression in HCT116/L-OHP cells compared with its parental cells. MRP-2 (MDR-associated protein 2) is an important MDR protein in platinum-drug-resistance cells and is a predicted target of miR-297. Additionally miR-297 was down-regulated in a panel of human colorectal carcinoma tissues and negatively correlated with expression levels of MRP-2. Furthermore, we found that ectopic expression of miR-297 in MDR colorectal carcinoma cells reduced MRP-2 protein level and sensitized these cells to anticancer drugs in vitro and in vivo. Taken together, our findings suggest that miR-297 could play a role in the development of MDR in colorectal carcinoma cells, at least in part by modulation of MRP-2.

Biography:

Songul Cetik_­­­ has successfully completed her Master’s and PhD from Eskisehir Osmangazi University in 2011 & 2014 respectively. She studies on topic: Phytotherapy on Side Effects of Chemotherapy (Toxicology). She is working as Assistant Professor in the Vocational Higher of Health Services unit.

Abstract:

Hypericum triquetrifolium Turra. (HT) is a phenolic component with anti-oxidative, analgesic and anti-carcinogenic properties. This study aimed to investigate the possible protective effect of HT on CP-induced hepatotoxicity. In order to determine the protective effect of HT upon the liver, the levels of Aspartate transaminase (AST), Alanine transaminase (ALT), Alkaline phosphatase (ALP) and Lactate dehydrogenase (LDH) as well as the total levels of Total Anti-oxidant Capacity (TAC) and Total Oxidant Capacity (TOC), were determined. Also, Oxidative Stress Index (OSI) was measured. Furthermore, the liver tissues were analyzed histologically. Albino rats (Wistar, 3-4 months old, male, weight 220 ± 20 g healthy) were randomly divided in 9 nine groups, each including 7 animals: Group 1 (control) treated with saline, Group 2 treated with 150 mg/kg CP, Group 3, 4 and 5 treated with 25, 50 and 100 mg/kg HT respectively, Groups 6, 7 and 8 treated with 25, 50 or 100 mg/kg HT+CP respectively, and Group 9 treated with 0.5 ml of %0.2 DMSO. The results were analyzed by One Way Analysis of Variance and Kruskal-Wallis One Way Analysis of Variance on Ranks Test. Our study demonstrated that in line with the rise in the dose of 150 mg CP, we determined an increase in the levels of serum ALT, AST, ALP, LDH, TOS and OSI, besides degeneration in the liver tissue. With 25, 50 and 100 mg/kg HT, there was an important decrease with respect to CP toxicity. In the groups given both CP plus HT, there was a rise in serum Total Anti-Oxidant Status (TAS) levels, while the levels of AST, ALT, ALP, LDH, TOS and OSI showed a remarkable decrease. The signs of recovery in the serum biochemical levels were also true for the histological findings of the liver. Our data suggest that HT is a highly effective antioxidant substance with a cell-protecting effect. Therefore, HT could serve as effective agent that could lessen the adverse effects of anti-cancer drugs in the course of chemotherapy protocols.

Biography:

Anna Morozova is working on her PhD project in the Department of Basic and Applied Neurobiology, V P Serbsky Federal Medical Research Center for Psychiatry and Narcology. She is also a Medical Doctor (Psychiatrist) in a mental hospital named Gilyarovskiy. She has published more than 10 papers. The last paper deals with a new approach in modeling of depressive-like behavior in rats and mice. She also studied the Ultrasound of alternating frequencies cause variable emotional impact which evokes depressive syndrome in mice and rats.

Abstract:

Emotional stress is primarily triggered by the cognitive processing of negative input; it is regarded as a serious pathogenetic factor of depression that is challenging to model in animals. While available stress paradigms achieve considerable face and construct validity in modeling depressive disorders, broader use of naturalistic stressors instead of the more prevalent models with artificial challenges inducing physical discomfort or pain may substantially contribute to the development of novel antidepressants. Here, we investigated whether a three-week exposure of Wistar rats and BALB/c mice to unpredictably alternating frequencies of ultrasound between the ranges of 20–25 and 25–45 kHz, which are known to correspond with an emotionally negative and with a neutral emotional state respectively, for small rodents in nature can induce behavioral and molecular depressive like changes. Both rats and mice displayed decreased sucrose preference, elevated despair behavior in a swim test, reduced locomotion and social exploration. Rats showed an increased expression of SERT and 5-HT2A receptor, a decreased expression of 5-HT1A receptor in the prefrontal cortex and hippocampus, diminished BDNF on gene and protein levels in the hippocampus. Fluoxetine, administered to rats at the dose of 10 mg/kg, largely precluded behavioral depressive-like changes. Thus, the applied paradigm of emotional stress is generating an experimental depressive state in rodents, which is not related to any physical stressors or pain. In essence, this ultrasound stress model, besides enhancing animal welfare, is likely to provide improved validity in the modeling of clinical depression and may help advance translational research and drug discovery for this disorder.

Biography:

Luciana Lopes Guimarães has completed her PhD from Federal University of São Paulo (Brazil) and Post-doctoral studies from the same Institution. She is an Adjunct Professor at Santa Cecília University (Brazil). She has published papers in reputed journals and has been serving as a Reviewer for Frontiers in Physiology, Frontiers in Microbiology and also for Medicine (Baltimore).

Abstract:

Special attention has been given to environmental contamination related to pharmaceuticals compounds because these pollutants, so-called emerging, may represent biological-ecological risk, especially in aquatic ecosystems. Antihypertensive drugs are used worldwide with evidence of their occurrence in environmental matrices and domestic effluents. This study aimed to investigate the occurrence of Losartan and Valsartan in a Brazilian coastal zone and also evaluated their adverse biological effects on sea urchin Lytechinus variegatus. Seawater samples were collected from six points surrounding the submarine outfall of Santos (São Paulo, Brazil) and the samples were analyzed by LC/MS/MS. The analysis revealed the presence of both Losartan and Valsartan with concentrations up to 0.024 µg.L-1. Acute toxicity assays were conducted according to U.S. Environmental Protection Agency protocol. Both Losartan and Valsartan exhibited IC₅₀ above 100 mg.L^-1 and these results classify these pharmaceuticals as "Non Toxic" according to directive 93/67/EEC, which classifies substances according to specific toxicity results. Chronic toxicity assays were performed according to Brazilian National Standards Organization protocol. Losartan exhibited values of NOEC (No Observed Effect Concentration) of 50 mg.L^-1 and CEO (Concentration Effect Observed) of 70 mg.L^-1. The results of NOEC and CEO for Valsartan were 9.37 mg. L^-1 and 18.75 mg L^-1, respectively. Differences of NOEC and CEO values for Losartan and Valsartan may be explained by the differences of Kow (octanol-water partition coefficient) values for these two molecules. Despite the occurrence of Losartan and Valsartan in seawater samples, it’s unlikely the occurrence of ecotoxicological effects, considering the environmental concentrations detected for these pharmaceuticals.

Biography:

Melike Ertem is a Research Assisstant at Psychiatric Nursing Programme. She completed her Master’s education at Duzce University. Currently, she is studying her PhD at Dokuz Eylül University Psychiatric Nursing Department. She voluntarily gave psychological counselling services at Elder Health Center in Bolu. She studied at Europen Union Grundving Life Long Lerning Project which named "Training and Psyco-Social Therapy for Informal Caretakers of Bedridden Disabled Individuals".  She has been studying on motivational interviews with schizophrenia diagnosed patients. She has published papers in reputed journals and oral –poster presentations at conferences.   

Abstract:

Medication nonadherence decreases the success of clinical treatment and the efficient use of resources, thereby creating a barrier to effective health care. In this report, we describe the achievement of treatment collaboration through motivational interviews (MI) in a patient with treatment-resistant schizophrenia. MI interventions are based on individual-centered counseling, cognitive-behavioral therapy, social-cognitive theory, the health belief model and the trans-theoretical model. In this case study, we conducted six MIs during which we asked open-ended and reflective questions, established empathy with the patient, and developed discrepancies, leading to ambivalent feelings being revealed. Each interview was structured in accordance with the principles of MI.  In this context, The Morisky Medication Adherence Scale and a questionnaire were used to assess his medication adherence. We used the importance, confidence and self-efficacy ruler. The MI method can be used to ensure continued treatment effectiveness, to increase patient awareness about the disease and benefits of treatment, and to increase patients' self-efficacy. MI can be included during any of the patient visits to establish treatment collaboration with patients as long as the provider has experience with limiting the length of the interaction at psychiatric facilities, outpatient clinics and community mental health centers. It can take months or years for people to experience a behavior change. MI techniques can encourage patients to think about their medication-taking behaviors in a different way, and after each short session they may be able to improve awareness and self efficacy.

This study was published at Archives in Psychiatric Nursing

Biography:

Kecheng Lei is pursuing her Doctor's degree in Jianwen Liu’s Lab. She has published a paper in Tumor Biology in 2015.

Abstract:

Epigenetic mutations are closely associated with human diseases, especially cancers. Among them, dysregulations of histone deacetylases (HDACs) are commonly observed in human cancers. Recent years, HDAC inhibitors have been identified as promising anticancer agents; several HDAC inhibitors have been applied in clinical practice. In this study, we synthesized a novel N-hydroxyacrylamide-derived HDAC inhibitor, I-7ab, and examined its antitumor activity. Our investigations demonstrated that I-7ab exerted cytotoxicity toward and inhibited the growth of human cancer cell lines at micromolar concentrations. Among tested cells, HCT116 was the most sensitive one to the treatment of I-7ab. However, I-7ab displayed far less cytotoxicity in human normal cells. In HCT116 cells, I-7ab inhibited the expression of class I HDACs, especially that of HDAC3 and suppressed EGFR signaling pathway. With respect to the cytotoxic effect of I-7ab, it induced apoptosis via increasing the Bax/Bcl-2 ratio and suppressing the translocation of NF-kB. Other than inducing apoptosis, I-7ab inhibited the expression of cyclin B1 and thereby arrests cell cycle progression at G2/M phase. Further analyses revealed potential role of p53 and p21 in I-7ab-induced apoptosis and cell cycle arrest. According to our findings, I-7ab may serve as a lead compound for potential antitumor drugs.

Biography:

S Al-azzawi a 3^rd PhD Student from School of Pharmacy and Bimolecular Sciences at University of Brighton, UK

Abstract:

Alzheimer's disease (AD) is a neurodegenerative disease caused by plaque accumulation of abnormal deposits of Amyloid-β (Aβ) in the brain. AD is considered epidemic with 33.9 million people worldwide suffering from it. The development of drugs to combat AD is hampered due to the presence of the Blood-Brain Barrier (BBB). Receptor mediated transcytosis is one of the promising strategies to deliver molecules with low BBB permeability by utilizing natural transport route. This project focuses on the innovative combination of ApoE-derived peptide integrated to a dendronized-drug conjugate which has the potential for improved brain endothelial uptake and targeting. ApoE-derived peptide was found to act as a ligand that can be recognized by lipoprotein receptors which are widely expressed in brain endothelium. ApoE-derived peptide and dendron-based carrier system were synthesized using solid phase peptide method by microwave peptide synthesizer. This carrier system was loaded chemically with Flurbiprofen which is one of poorly permeable AD drugs that decrease Aβ. Immortalized brain endothelial cell has been used as a model for BBB. This study has demonstrated the successful designing, synthesis and functionalization of a biocompatible drug carrier system with the potential to act as novel carrier for improved targeting and crossing the BBB.

Biography:

Sopio Gokadze has graduated from Tbilisi State Medical University (TSMU). She has obtained her MS degree in Pharmacy in 2012. She worked at Scientific Research and Practical Skills Laboratory. Currently, she is a PhD Student at TSMU Department of Pharmaceutical Technology. She is also a co-author of 5 scientific publications and 3 presentations at international conferences. 

Abstract:

A high molecular (> 1000 KDa) water soluble biopolymer WSCP was obtained from the roots and stems of two Caucasian species of comfrey S. asperum and S. caucasicum. The biopolymer appeared to have diverse pharmacological activity including anti-inflammatory, antioxidant, anticancer and wound healing. The aim of present study was an attempt to choose optimal carriers and excipients for novel ointment on the basis of biopharmaceutical investigations. Eight compositions with various combinations of carriers and excipients (petroleum jelly, anhydrous lanoline, distilled water, sodium carboxymethylcellulose, polyethylene glycol -1000, glycerin, sodium alginate, askana clay, and Tween-80) were designed and studied for: Determination of optimal ointment base, establishment of ointment stability, and biopharmaceutical study. Stability of ointment was evaluated using centrifugation (6000 rpm for 5 min) and thermal assays. Compositions 1, 2, 3 and 6 passed the test, whereas in compositions 4, 5, 7 and 8 the stability was slightly affected by centrifugation. The biopharmaceutical study was carried out by using the agar diffusion assay. Based on the obtained results the optimal ointment formulation has been justified and formulated WSCP is with the combination from S. asperum, distilled water, and sodium alginate. Biopharmaceutical studies established that the maximal release of WSCP from the ointment was achieved when sodium alginate was used as ointment base.