35^th World Congress on Pharmacology August 01-02, 2022 Rome, Italy

Biography:

Dr Kelleni is also the inventor and one of the right holders of two internationally protected IP medical project.

Abstract:

Early management of COVID-19 is of crucial importance as agreed by experts all over the world. However, there’s no consensus regarding the best protocol that should be adopted. Kelleni’s protocol to manage COVID-19 is based on numerous publications made by the author in reputable international medical journals since April 2020, it’s been safely and effectively used to manage pediatric, adult, geriatric and pregnant COVID-19 patients suffering from mild – severe disease and in this case report, the author suggests that hepatitis/pancreatitis of unknow origin should be thoroughly investigated as a potential novel SARS CoV-2 variant should not be easily excluded and a potential COVID-22 should also be kept in the differential diagnosis in order to avoid repeating the previous mistakes that turned an outbreak into a pandemic that harvested millions of innocent lives. A female in her late seventh decade with a history of chronic hepatitis B viral infection and moderately reduced kidney function suffered a syndrome of severe headache, acute epigastric abdominal pain, nausea, vomiting, confusion, and insomnia that could have been attributed to pancreatitis. However, oxygen saturation, CT chest and laboratory investigations showed evidence of severe COVID-19 while she suffered silent hypoxia and didn’t complain of cough, sore throat, or fever. She was safely and effectively managed using personalized expanded Kelleni’s protocol for three nights only in hospital and she continued treatment at home with over a month follow up until full recovery. We call for routine oxygen saturation screening by pulse oximeter for all high-risk patients currently seeking medical advice for any reason and I suggest if the current criteria to diagnose pediatric hepatitis of unknown origin is modified to include patients with ALT or AST level lower than the currently adopted 500 IU/L, a potential mountain beneath iceberg could be better revealed and examined.

Biography:

Marco Milanese is Associate Professor at the Department of Pharmacy of the University of Genoa. Since 2005 Marco Milanese is member of the Pharmacology and Toxicology research Unit of the Department of Pharmacy, University of Genoa, working in the field of neuropharmacology and neurosciences. Marco Milanese holds a long-lasting experience in training undergraduate students and PhD students other than managing a biological research laboratory with a broad panel of expertise and facilities. The scientific research of Marco Milanese started focusing on various aspects of neurotransmission and relative molecular mechanisms underlying neurological disorders and neurodegenerative diseases: release of neuro- and glio-transmitters and modulation systems, pre-synaptic proteins, pharmacological characterizations of pre- and postsynaptic glutamatergic receptors. In this context the research activity of M. Milanese was mainly focused on the study of glutamatergic neurotransmission linked to excitotoxicity in neurodegenerative diseases with particular interest on motor neuron diseases (MND) and amyotrophic lateral sclerosis (ALS). In the last ten years the research activities of M. Milanese were characterized by in-vivo behavioural analysis and ex-vivo histological studies to assess the clinical progression and the molecular dysfunction in mouse model of ALS and/or other neurological disorders. The areas of investigation, in the context of ALS, have then expanded towards complementary in-vitro functional and biochemical studies on motoneuron, astrocyte, microglia and oligodendrocyte primary cell cultures acutely isolated from ALS mouse models. M. Milanese holds active scientific collaborations with National and International research groups, as intelligible from the publication track record.

Abstract:

Amyotrophic lateral sclerosis (ALS) is a progressive, fatal, neurodegenerative disease characterized by the selective death of upper and lower motor neurons (MNs). The mechanism of MN damage and death has been ascribed to several cellular and molecular alterations, including neuro inflammation. ALS is also a non-cell-autonomous disease, due to the contribution of glial cells, such as astrocytes and microglia that secrete neurotoxic factors and promote a noxious environment for MN. At present there is no effective cure for ALS. We previously demonstrated that intravenous administration of bone marrow mesenchymal stem cells (MSCs), prolonged survival probability, improved motor functions and ameliorated pathological features, including gliosis and neuro inflammation, in the spinal cord of the SOD1G93A mouse model of ALS. There is a consensus supporting that the beneficial outcomes of MSCs are unlikely due to trans-differentiation, but possibly to paracrine effects, thus we postulated that one of these mechanisms could be the transfer to target cells of microRNAs (miRNAs) shuttled by extracellular vesicles (EVs) isolated from the secretome of MSCs. To this aim we studied the activity of MSCs-derived EVs both on astrocytes isolated from the spinal cord of symptomatic SOD1G93A mice and human astrocytes (iAstrocytes) differentiated from inducible neural progenitor cells (iNPCs) of ALS patients.

Biography:

Qian Yin major courses include neurobiology, principles of neuroscience, molecular cell biology and cell electrophysiology experimental technology. During this period, She was mainly engaged in calcium imaging experiment, electrophysiological patch clamp experiment, molecular biology experiment, cell culture and rat behavior experiment, mouse nerve ligation operation, etc. Master electrophysiological patch clamp technique and record Na + channel current, TRPV1, ASIC3 receptor channel current. Master cell calcium imaging technology, immunofluorescence technology, ELISA experiment, Western blot, etc. Master rat and rat pain model, cooperative sleep model and related animal behavior experiments, mouse nerve ligation, etc. Master SPSS, Graph pad, PS, Image J and other software, and have good statistical analysis ability of experimental data. She participated in the research on the "analgesic mechanism of total saponins and their components regulating substance P and its receptor (NK-1)" of the graduate academic innovation fund project. At present, She is participating in the research of "sedative hypnotic mechanism of Sabina vulgaris"

Abstract:

Pain is one of main problems endangering human health. The research and development of new analgesic drugs with high efficiency, small side effects and no addiction has always been a hot research direction. Stauntonia chinensis is a traditional Chinese medicine and has been made into a variety of preparations. Acupoint injection of Stauntonia chinensis injection can produce a good clinical analgesic effect. Studies have shown that the saponins of Stauntonia chinensis may be the analgesic effective part. In our studies, the triterpenoid saponins from Stauntonia chinensis (TSS) were successfully extracted by a flash extraction way and the basic skeleton structures were identified. Through in vivo animal experiments and acute pain model, we found that TSS had short-term analgesic effect. In the formalin test, the time spent in licking the injected paw in phase II could be significantly decreased by TSS. In the capsaicin test, TSS also could decrease the time spent in licking the injected paw caused by capsaicin. Using the rat model of inflammatory pain induced by complete Freund's adjuvant (CFA), we also found that TSS had long-term analgesic effect. With the patch clamp technique and Western blot technique, we further observed the interaction between TSS and TRPV1 receptor, which is closely related to pain production and conduction. We found that TSS could inhibit the capsaicininduced TRPV1 current. Compared with the control group, the expression of TRPV1 in DRG neurons of CFAinduced inflammatory pain rats could be increased and TSS could reduce the increased protein expression of TRPV1. The above results showed that the analgesic effect of TSS may be related to blocking TRPV1 receptor and interfering with its protein expression, which will provide an important pharmacological reference for the development of new analgesic drugs from Stauntonia chinensis.

Biography:

It is widely accepted that surfaces of the vasculature express varying tissue-specific receptors under different pathological conditions1. Oh et al. used subtractive proteomics analysis of malignant vs. normal vasculature to identify Annexin A1 (ANXA1) as a highly specific surface marker of malignant tumor vasculature 2, 3. Independently, in mouse studies we reported that IF7 peptide, IFLLWQR, which binds the ANXA1 N-terminus, functions as a tumor vasculature-targeted drug delivery vehicle after intravenous injection4-6. To enhance IF7 stability in vivo, we undertook mirror-image peptide phage display using a synthetic D-peptide representing the ANXA1 N-terminus as target. We then identified candidate L-peptide sequences, synthesized them using D-amino acids, and found that one peptide bound to the L-ANXA1 N-terminus. We designated that peptide dTIT7. When we injected mouse brain tumor

Abstract:

Michiko N.Fakuda has her expertise in evaluation and passion in improving the health and wellbeing. Her open and contextual evaluation model based on responsive constructivists creates new pathways for improving healthcare. She has built this model after years of experience in research, evaluation, teaching and administration in education institutions which is a methodology that utilizes the previous generations of evaluation: measurement, description and judgment. It allows for value-pluralism. This approach is responsive to all stakeholders and has a different way of focusing.

Biography:

Graciela Mahler, PhD, Professor of Organic Chemistry was born in Paysandú Uruguay. She Started out in education for Chemistry for some years. Bachelor of Science, Chemistry – 1992, Universidad de la República (UdelaR), Montevideo, Uruguay. 2003 she got her PhD in organic chemistry, Montevideo UdelaR and in 2004 was a pos doc at the Chemistry Department, University of Pittsburgh. Since 2005 is a professor at the University of the Republic. Over the last 17 years her main scientific interest includes the development of enzyme inhibitors using different tools like: dynamic combinatorial chemistry, bioisosterism and virtual screening. She focuses on the development of new synthetic methodologies for the rapid generation or molecular complexity to evaluate it bioactivity against trypanosomatids, helmints, viruses and bacteria. Awards: Researcher level II of the National Agency of Research and Innovation (ANII) (2015 to present), Member of the PEDECIBA council (2021 to present), Thieme Chemistry Journal Award (2013).

Abstract:

Severe acute respiratory syndrome β-coronavirus 2 (SARS-CoV-2) is the causal agent of the coronavirus disease first reported in 2019 (COVID-19). It is the third epidemic triggered by a coronavirus that evolved in recent years after the spread of SARS-CoV in 2002, and Middle East respiratory syndrome (MERS-CoV) that spread in 2012. SARS-CoV-2 produces a spike protein that binds to host cell receptor ACE2 for entry.[1] Upon entry, the positive genomic RNA of SARS-CoV-2 will attach directly to the host ribosome and translate two large polyproteins, which are then processed by proteolysis into components for packaging new virions. This proteolysis is controlled by two protease enzymes, the coronavirus main protease (Mpro) and the papainlike protease (PLpro). These proteins are essential for viral replication, they are considered attractive drug targets for treating coronaviruses.

Biography:

Abstract:

In this study, we applied classical molecular dynamics (MD) simulations to understand the structure, dynamics and transport properties of the room-temperature 1-alkyl-3-methylimidazolium family with tris(perfluoroalkyl)trifluorophosphate ionic liquids, abbreviated as [Cnmim][eFAP]. Calculated densities of ionic liquids agree well with experimental data. Local structures were characterized by studying the centerof- mass (COM) and partial site-site RDFs, combined distribution functions (CDFs) and dihedral angles distribution of n-alkyl side chains in the imidazolium cations. The hydrogen bonding between Fluor atoms of anions and hydrogen atoms of imidazolium ring of cations were studied by contour maps of CDFs. Also, dynamical properties of these ILs such as mean-square displacement (MSDs) for center-of-mass of ions, ionic diffusion coefficients and the cationic transference numbers are calculated by simulation in the NVT ensemble. Results show that the length of alkyl-side chain of cation is the major factor to affect these properties.

Biography:

Antonio Steardo specialized in Pharmacology and graduated in Pharmacy and Pharmaceutical Chemistry. He has now gained years of experience since 2002 in the pharmaceutical products trade sector as he could have been behind the counter of the Steardo pharmacy from an early age. Already in elementary school, the curiosity for chemistry manifests itself during his games and continues lectures at the department of science at the University of Salerno. Therefore, during the cycle of studies, he prefers biochemistry and biochemistry of drug action, graduating in July 2007 with a thesis on the functioning of the endocannabinoid system on Alzheimer’s disease in pharmacology. Following the beginning of his pharmaceutical chemistry studies, he stopped for a competition as a postgraduate in pharmacology at the University of Rome La Sapienza in July 2014. Expecting constant improvement as a professional update, he enrolled in the continuing professional training department at the University of Oxford to follow courses in Experimental and Translation Therapy and on Medical Research. His desire to improve leads him to attend international conferences and seminars.

Abstract:

The key role of the immune response appeared to be related to Tool-Like receptors since the early stages of the studies conducted on Covid-19. Nowadays the robust evidence emerged in literature seem to confirm more than what it has been hypnotised. The key role of Tool-Like receptors act causing by within the infection, a storm by cytokines such as interleukin‐1 (IL‐1), IL‐6, and tumour necrosis factor‐α, as well as type 1 interferon. The whole pathway causes internal organs damage resulting not only on long sequala but even on persistent tissue damages. As passed study Alia-amides like Palmitoyl-ethanol-amide demonstrated the capability of Tool-Like receptor for aberrant tissue damage mediators. As it has yet demonstrated on Ulcerative Colitis. Would it be the change to set test to assess Alia-Amides properly and block Tool-Like pathways dependent tissue damage due to Coronavirus infection by the Tool-Like receptor mechanism disease related?

Biography:

Abstract:

Traditional medicine has been widely used for healing of diseases, as an alternative to modern medicine. Maldivians rely on traditional medicine (“Dhivehi beys”) such as “Thakoo sarubathu”, formulated as a cure for children having diarrhea and vomiting. The two main ingredients of Thakoo sarubath include liquorice (Glycyrrhiza glabra linn) and Saffron (Crocus satvus) that have many health benefits such as antifungal, antiviral and antibacterial properties. The aim of this study was to assess the adulterants, microbial and heavy metal contaminants in thakoo sarubathu. Thakoo sarubath was purchased from local traditional medicine shop in Maldives and analysis was carried out at SGS Lanka Laboratory Pvt Ltd, Sri Lanka. Modern medicine adulterants including aspirin and paracetamol was analyzed using near infrared spectroscopy. Heavy metals were analyzed using atomic absorption spectroscopy. Microbial analysis was carried out using accredited methods. The results revealed that none of the adulterants of modern medicine was detected. Also, heavy metals including Pb, As, Cd, Cr, Cu, Tl, Mn and Hg were not detected. The aerobic bacteria and coliforms were within the acceptable limit and Salmonella spp was not detected. Overall, results indicate that Thakoo sarubathu is safe for children to consume.