14^th World Congress on Pharmacology and Toxicology September 11-12, 2019 Singapore

Biography:

D S Mohale has completed PhD from PRIST University and M Pharm from RTMNU. He is currently working as an Associate Professor in Pharmacology at P. Wadhwani College of Pharmacy, Yavatmal with 13 years of teaching experience. He published 29 research and review articles in reputed journals.

Abstract:

Objective: The current study was designed to determine the effect of acetate and Selective Serotonin Reuptake Inhibitors (SSRI) on stress and blood glucose level in stressed rat.

Method: Animals were divided in 5 groups (n=6) , treated with 20 mg/kg p.o. of Fluoxetine (SSRI) and Glyceryl Triacetate (GTA) at 6 g/kg p.o dose (acetate supplementation) alone and in combination for 28 days to assess its effect in immobilized stressed rats. Open field and hole-board test were used for determination of stress in animals followed by blood glucose level.

Results: Animals treated with 20 mg/kg p.o Fluoxetine (SSRI), 6 g/kg p.o GTA and SSRI (Fluoxetine) + GTA showed significant (p<0.01) stress resistant activity as compared to negative control. Results also demonstrated that there was significant (p<0.01) decrease in blood glucose level in animals treated with 20 mg/kg p.o Fluoxetine (SSRI), 6 g/kg p.o GTA and SSRI (Fluoxetine) + GTA.

Conclusion: It concludes acetate and SSRI supplementation posse’s stress resistant and hypoglycemic potential in immobilized stressed rats by enhancing the acetylation of histone.

Biography:

These results envisage that Nevirapine mucoadhesive microspheres improve perfusion to the ischemic region of the brain and revealed to its antioxidant and neuroprotective activity. Further studies are needed to confirm its molecular mechanism involved in neuroprotection and repurposing of Nevirapine.

Abstract:

Objective: To evaluate the neuroprotective effect of mucoadhesive microspheres of Nevirapine on cerebral ischemic stroke by middle cerebral artery occlusion in Wistar rats.

Method: Evaluation of Nevirapine mucoadhesive microspheres by particle size, morphology, drug entrapment efficiency, in vitro wash off test, in vitro drug release and interaction studies include Fourier Transfer Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC). The rats were pre and post treated with mucoadhesive microspheres of Nevirapine at selective doses (5, 10 mg/ kg/g, p.o) for a period of 14 days for evaluation of neuroprotective property in Middle Cerebral Artery Occlusion (MCAO) induced cerebral ischemic rats. Neurobehavioral changes were evaluated by using Y maze and open field habituation. Biochemical markers such as Acetyl Cholinesterase (AChE), glutamate, Differential Leukocyte Count (DLC), Lactate Dehydrogenase (LDH), antioxidants such as Superoxide Dismutase (SOD), Glutathione Peroxidase (GP_x) and catalase were estimated.

Results: Optimized formulation of Nevirapine mucoadhesive microspheres provided controlled delivery and prolonged residence at the absorption site. Obtain results revealed that 14 days of treatment with Nevirapine microspheres was effective forefend ischemia induced neurotoxicity. Nevirapine microspheres treatment decreases AChE, glutamate, DLC, LDH and increases the antioxidant parameters such as SOD, catalase and GP_x.