24^th World Congress on Pharmacology August 19-20, 2019 Vienna, Austria

ABSTRACT Introduction: Attention Deficit Hyperactivity Disorder (ADHD) is a common mental disorder in children. Drug treatment is the most prevalent method used to control it; however, considering the low efficacy and frequent side effects of current drugs, more attempts are needed to replace them with safer agents. Several studies have shown the beneficial role of micronutrients such as vitamin D in development and improving the performance of neuronal system. This research intended to study the effects of vitamin D supplementation in 6–13 year-old students with ADHD. Methods: In this double-blind parallel clinical trial, the subjects were selected from among 6–13 year-old students with ADHD diagnosed by a child psychiatry specialist. Vitamin D3 supplements (1000 IU) or placebo given daily to 70 subjects for three months. ADHD symptoms were evaluated before and after the intervention using Conners Parent Questionnaire (CPQ), the Strengths and Difficulties Questionnaire Teacher Version (SDQT), the Strengths and Difficulties Questionnaire Parent Version (SDQP) and Continuous performances Test (CPT) scores. Results: The mean scores of the CPQ, SDQP and SDQT showed a significant difference in the two groups after intervention (p < 0.05). The impulsivity mean scores of the CPT after intervention showed statistical significance (p =0.002), but the attention (p =0.11) and mean reaction time (p =0.19) mean scores did not. Conclusions: Vitamin D supplementation not only improves some behavioral problems but may prevent exacerbation in some symptoms of the disorder and reduce impulsivity.

Fouad Azizi has earned his PhD in Biophysical Chemistry at the Center of Molecular Biophysics, National Center for Scientific Research (C.N.R.S.), Orleans, France. He has a research track of over 20 years working in the USA. Currently, he is a Research Scientist, Director of electrophysiology laboratory and Manager of confocal imaging core in the Translational Research Institute, Academic Health System, Hamad Medical Corporation (HMC), Doha, Qatar. His research interests are focused in cancer and metabolic disorders.

Fouad Azizi has earned his PhD in Biophysical Chemistry at the Center of Molecular Biophysics, National Center for Scientific Research (C.N.R.S.), Orleans, France. He has a research track of over 20 years working in the USA. Currently, he is a Research Scientist, Director of electrophysiology laboratory and Manager of confocal imaging core in the Translational Research Institute, Academic Health System, Hamad Medical Corporation (HMC), Doha, Qatar. His research interests are focused in cancer and metabolic disorders.

Yosra BEDOUI obtained her degree of doctor of pharmacy in 2014 at Monastir University, Tunisia. She is currently in her third year of her PhD at the University of La Reunion, France. She is working at the UMR PIMIT. Her thesis is devoted to the evaluation of the immunomodulatory and anti-viral role of methotrexate in the context of chronic arthritis induced by Chikungunya virus infection. Her interests are focused on the study of the physiopathological mechanisms of chronic arthritis induced by Chikungunya virus and the immunomodulatory effects of methotrexate treatment.

Abstract Methotrexate (MTX), the first line disease modifying anti-rheumatic drug in rheumatoid arthritis (RA) therapy, has been used successfully to treat patients with rheumatoid-like arthritis post-Chikungunya virus (CHIKV) infection (1). However, mechanisms by which MTX exerts its therapeutic effect are poorly understood. The eicosanoid prostaglandin (PG) E2 is one of the most important mediators of inflammation and contributes to several pathogenic features of arthritis such as pain and bone destruction (2,3). The proinflammatory cytokines IL-1β and TNFα, which play a pivotal role in initiating and driving RA, are known to enhance PGE2 production (4). We herein used a model of primary human synovial fibroblasts (HSF) infected with CHIKV or stimulated by the synthetic molecule polyriboinosinic:polyribocytidylic acid (PIC) to mimic chronic viral infection (5) and assessed the potential pharmacological effects of MTX. By quantitative RT-PCR, we found that PIC but not CHIKV increased the mRNA level of group IVA cytosolic phospholipase A2 (cPLA2α) (6) , a central enzyme in AA-derived eicosanoid production. Similarly, PIC but not CHIKV upregulated mRNA expression of the microsomal prostaglandin E2 synthase 1 (mPGES-1) (7) enzyme involved in PGE2 synthesis. In contrast, we found that PIC and CHIKV enhanced mRNA expression of cyclooxygenase 2 (COX-2) (4) , a major PGE2 biosynthetic enzyme. Moreover, PIC and CHIKV decreased mRNA expression of the PGE2 degrading enzyme 15-hydroxyprostaglandin dehydrogenase (15-PGDH) (8) and this effect was not modulated by MTX. As controls, we found that IL-1β as well as TNFα stimulated mRNA levels of PLA2, COX-2, mPGES-1 and all these effects were inhibited by dexamethasone (DXM). DXM, in contrast, upregulated mRNA expression of the PGE2 degrading enzyme (15-PGDH). These original data argue for a therapeutic activity of MTX independently of PGE2 regulated response and through novel mechanisms which remain to be explored.

Biography Maannashon Prabaharan is an intercalating student who has interests in pharmacology, and its uses in clinical practice. This paper vocalizes his opinions about the current clinical scenario regarding sepsis (Levy et al. 2018), and potential future changes that could be implemented in all aspects of clinical care.

Abstract Sepsis is caused by the host’s over-response to an infection, which leads to organ failure. This affects many areas of the body, including the cardiovascular, renal, GI and pulmonary systems. Sepsis has high mortality rates, with survivors being affected by complications, including cognitive decline and increased cardiovascular events. Current methods for diagnosing sepsis include the use of physical biomarkers such as heart rate (HR), and serological biomarkers such as C-Reactive Protein (CRP) and Procalcitonin (PCT). Clinical trials were found through literature searches using the PubMed and Ovid databases. The cumulative evidence suggests that other serological biomarkers such as presepsin, Pentraxin-3 (PTX3) and micro-RNA have potential for future clinical use. Heart rate variability (HRV) is a newer physical biomarker that has good evidence for diagnosing sepsis patients. The Surviving Sepsis Campaign has annual updates on guidelines for clinicians in treating sepsis. The latest guidelines have included the empirical use of broad-spectrum antimicrobials to be given immediately, as part of a ‘1-hour bundle’, which does have disadvantages. The growing evidence suggests of a trend in increasing antimicrobial resistance, therefore, new alternatives should be found. This text has evidence for alternative methods, such as the use of antimicrobial stewardship (responsible use of antibiotics) and bacteriophages (viruses which infect and destroy bacteria). Recent innovations in technology over the past decade have been integrated into clinical practice, and there is great hope for the near future with new research into predictive algorithms and consumer technology in treating patients. This review aims to summarise the current developments that have occurred in the diagnosis, treatment and management of septic patients. This review also aims to show the reader what future developments hold for improving the quality of sepsis management.