Sleman Kadan
Al-Qasemi Academic College, Israel
Title: Gundelia tournefortii anti-diabetic efficacy: Chemical composition and GLUT4 translocation
Biography
Biography: Sleman Kadan
Abstract
In the present in vitro study, we tested the chemical composition, cytotoxicity and anti-diabetic activity of two wild artichoke-like vegetable, Gundelia tournefortii, and distinct extracts: methanol and hexane. GC/MS phytochemical analysis of Gundelia tournefortii methanol and hexane extracts revealed 44 compounds reported here for the first time in Gundelia tournefortii. Out of the 50 detected compounds only stigmasterol was present in both extracts. The efficacy of Gundelia tournefortii extracts in enhancing glucose transporter-4 (GLUT4) translocation (fig. 1) to the plasma membrane (PM) was tested in L6 muscle cells stably expressing myc-tagged GLUT4 (L6-GLUT4myc) using cell-ELISA test. Results obtained here indicate that methanol and hexane extracts were safe up to 250 µg/ml as measured with MTT and the LDH leakage assays. The methanol extract was the most efficient in GLUT4 translocation enhancement. It increased GLUT4 translocation at 63 µg/ml by 1.5 and 2 folds relative to the control in the absence and presence of insulin, respectively. These findings indicate that Gundelia tournefortii possesses anti-diabetic activity in part by enhancing GLUT4 translocation to the PM in skeletal muscle.