Heba Abdel-Hady
Theodor Bilharz Research Institute, Egypt
Title: Characterization and evaluation of antioxidant activity of Ocimum canum leaves and its effeciency on Schistosoma mansoni larval stage
Biography
Biography: Heba Abdel-Hady
Abstract
Schistosomiasis is the most endemic disease caused by trematode worm of the genus Schistosoma. Its control is dependent on kill the worm itself or one of its larval stages. The objective of this study is determination of the total phenolic and flavonoid contents, evaluating the scavenging inhibitory effect of Ocimum canum leaf methanol extract on DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS as well as investigating the efficiency of plant extract against larval stages of Schistosoma mansoni. Also, chemical composition of plant extract was identified by GC-MS analysis. Antioxidant activity of the plant extract was evaluated by DPPH and ABTS scavenging assays while, cercaricidal and miracidicidal activity of it were tested at different concentrations directly on the larval stages for 2 hours, dead larvae were recorded each 30 minutes. The results revealed that Ocimum canum leaf methanol extract showed high total phenolic (321.78±0.69 mg GAE/g extract) and flavonoid contents (71.64±10.66 mg RE/g extract). Furthermore, there is a significant correlation between the concentration of the extract and the inhibition percentage of the free radicals. The higher inhibition percentage of plant extract was recorded by DPPH than ABTS at 500 µg/ml (84% and 62%) respectively; also, IC50 of DPPH and ABTS (26.41±1.39 and 194.45±2.03) respectively. Regarding to cercaricidal and miracidicidal activity, the plant extract showed larvicidal activity as it killed 82% of cercariae while, it was completely lethal to miracidia at 500 ppm within 2 hours. On the other hand, 36 chemical compounds were identified from plant extract. The main components are phytol (23%), hexadecanoic acid, methyl ester (15.48%), 9,12,15-Octadecatrienoic acid methyl ester (14.99%), n-Hexadecanoic acid (7.62%), 9,12,15-Octadecatrienoic acid (5.04%), ethyl linoleate (3.58%) and 2-propenoic acid, 3-phenyl-, methyl ester (3.17%). This study suggested that Ocimum canum can be used as cercaricidal and miracidicidal promising plant to control schistosomiasis disease as well as its potential use as antioxidant plant.