Illa C Patel
Hemchandracharya North Gujarat University, India
Title: HPTLC Method for studies of Phytochemicals from in vivo and in vitroDeveloped Plants of Pterocarpusmarsupium (Roxb.).
Biography
Biography: Illa C Patel
Abstract
Pterocarpusmarsupium Roxb. is an medicinally important plants belongs to the family Fabaceae commonly known as “Biyo” or “Indian Kino” distributed in forest of the Western Ghats of India.Plant contain various phytochemicals compounds likePhenol, flavonoids, alkaloids, Saponin, glycosides, stiroids etc. The main constituents arepterostilbene, triterpene alcohol, catechin, pterosuprin and marsupol which have medicinal properties to curing the different diseases likekapha and pitta, elephantiasis, erysipeals, rectalgia, dysentry, urinary discharge, piles and in diabetes. This plant urgently need to be conserve as it is highly exploited due to use of its heart wood and bark for obtaining many phytochemicals from it. Plant needed an ideal protocol for rapid multiplication and valid method of phytochemical analysis using atechniques of High Performance thin layer chromatography (HPTLC).This studies is aimed to developed in vitro callus and plants on MS media containing hormones viz.2,4-D, BAP, IAA individually at different concentration and in combination by using tissue culture techniques. The in vitro developed plant and callus were used to compare the phytochemicals constituent of it with in vivo plant parts through qualitative analysis in different extracts and quantify the amount of pterostilbene in eachpartwith using standard pterostilbene through HPTLC. Result of Priliminary analysis shows that in vitro developed callus contain many phytochemicals and HPTLC analysis shows it is a good source of pterostilbene. This study may be helpful for in vitro synthesis of phytoconstituents and give new resources to Pharma industry and also reduces pressure of natural plant population.
