Scientific Program

Conference Series LLC Ltd invites all the participants across the globe to attend 13th World Congress on Pharmacology and Toxicology Melbourne, Australia.

Day 2 :

Keynote Forum

Yukio Yoneda

Kanazawa University, Japan

Keynote: Stimulation of embryonic and adult neurogenesis by the green tea amino acid theanine

Time : 10:00-10:45

Pharmacology Congress 2018 International Conference Keynote Speaker Yukio Yoneda photo
Biography:

Dr. Yukio Yoneda is a Professor Emeritus in Kanazawa University, where he worked as a Chairman Professor of Pharmacology from 1999 to 2015. He graduated from Osaka University, Faculty of Pharmaceutical Sciences in 1972, followed by appointments in Kyoto Prefectural University of Medicine, City of Hope Research Institute, CA, USA and Setsunan University. He is serving as an Associate Editor in several international journals such as Neurochemistry International, Neurochemical Research and Journal of Neuroscience Research, in addition to acting on the editorial board in other international scientific journals. His research interests have been lying on pharmacological profiling of amino acid signaling in neuronal and non-neuronal cells using molecular biological techniques. As a return of laboratory experimental results to the community, he is now attempting to develop several dietary supplements beneficial for the prophylaxis of different diseases besides the bench work with colleagues in domestic and foreign universities.

Abstract:

Theanine is an exogenous amino acid in the green tea, rather than black tea and oolong tea, with a chemical structure analogous to several neuroactive endogenous amino acids such as glutamine and glutamate. We have been studying pharmacological profiles of this green tea amino acid featuring higher relevancy to glutamine than glutamate in neural progenitor cells capable of proliferating for self-replication and differentiating into neuronal, astroglial and oligodendroglial lineages in embryonic, developing and adult brains. Significant amelioration was found in cognition ability scores determined by double-blinded expert physicians in healthy elderly age-matched people given capsules of powdered green tea enriched of theanine compared to those with normal green tea powder capsules after daily oral intake for 7 to 12 consecutive months. In cultured neural progenitor cells isolated from embryonic rat and mouse neocortex, theanine promoted both proliferation and subsequent neuronal differentiation in a concentration-dependent manner, along with deteriorated astroglial differentiation. In cultured progenitor cells from the hippocampus of adult nestin-GFP mice, moreover, theanine increased the size of neurospheres composed of clustered proliferating cells after sustained exposure. In murine embryonic carcinoma P19 cells, similar promotion was seen in proliferation and neuronal differentiation after exposure to theanine. Exposure to theanine for a rather long time up-regulated the glutamine transporter Slc38a1 transcript expression in rat and mouse progenitors, whereas theanine failed to further promote both proliferation and neuronal differentiation activities already facilitated in P19 cells stably overexpressing Slc38a1. Theanine would be thus endowed to promote embryonic and adult neurogenesis through acting at neural progenitor cells in a manner related to upregulation of the glutamine transporter Slc38a1 in rodent brains. We have made several dietary supplement products enriched of theanine supposed to be beneficial for the prophylaxis of particular abnormalities in brain functions as a return of research benefit to the community. 

  • Sessions: Pharmacology; Toxicology; Drug Discovery & Drug Screening; Ethnopharmacology; Clinical Pharmacology & Receptor Therapy; Psychopharmacology & Neuropharmacology

Session Introduction

Nader G Abraham

New York Medical College, New York

Title: Translational significance of heme oxygenase in obesity and metabolic syndrome
Speaker
Biography:

Nader G Abraham is working in Departments of Medicine and Pharmacology at New York Medical College, New York

Abstract:

The global epidemic of obesity continues unabated with sequelae of diabetes and metabolic syndrome. This review reflects the dramatic increase in research on the role of increased expression of heme oxygenase (HO)-1/HO-2, biliverdin reductase, and HO activity on vascular disease. The HO system engages with other systems to mitigate the deleterious effects of oxidative stress in obesity and cardiovascular disease.  Recent reports indicate that HO-1/HO-2 protein expression and HO activity have several important roles in hemostasis and ROS-dependent perturbations associated with metabolic syndrome. HO-1 protects tissue during inflammatory stress in obesity through the degradation of pro-oxidant heme and the production of CO and bilirubin, both of which have anti-inflammatory and anti-apoptotic properties. In contrast, repression of HO-1 is associated with increases of cellular heme and inflammatory conditions including hypertension, stroke and atherosclerosis. HO-1 is a major focus in the development of potential therapeutic strategies to reverse the clinical complications of obesity and metabolic syndrome.

 

Speaker
Biography:

Dr. Hesham N. Mustafa has received his MD in basic medical sciences (Anatomy) from Ain Shams University, Cairo, Egypt at 2009. Currently, he is working as an associate professor in basic medical sciences (Anatomy) department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia. He has published more than 15 papers in reputed journals and been serving as an editorial board member and a reviewer at many reputed Journals. He received Certificate for Highly Cited Paper from Elsevier in December, 2016 And Award of Excellence of Scientific Publication for the staff members, from Deanship of Scientific Research, King Abdulaziz University.

Abstract:

Background. Renal ischemia-reperfusion injury (IRI) represents the main reason for acute kidney injury (AKI). Dexmedetomidine (Dex) and Benincasa cerifera (BC) have wide beneficial effects being anti-inflammatory and antioxidants. This study aims illustrate the protective effects of BC and Dex on renal IRI of diabetic model.

Methods. Sixty Male Albino Rats (Wistar strain), weighing 250-300 g. Rats were divided in to 4 groups; Sham group: Non-diabetic. DM+IRI group: STZ-diabetic rats exposed for renal IRI on days 30 after diagnosis of diabetes. DM+IRI+BC group: STZ-diabetic rats were treated with Benincasa Cerfera (500 mg/kg) for 30 days after diagnosis of diabetes then they exposed for renal IRI. DM+IRI+Dex group: STZ-diabetic rats were treated with Dex (100µg/kg intra-peritoneal) 5 minutes before induction of ischemia on day 30 after diagnosis of diabetes then they exposed for renal IRI. Biochemical parameters, histopathological examination and immunohistochemical markers were evaluated.

Results. Significant improvement of biochemical, histopathological and immunohistochemical parameters in (DM+IRI+BC) group while (DM+IRI+Dex) group showed an improving in renal IRI and dyslipidemia.

Conclusion. The present study demonstrated that oxidative stress plays a chief role in renal IRI in STZ-induced diabetic model. Treatment with BC achieved excellent ameliorative effects while treatment with DEX improves renal IRI.

 

Speaker
Biography:

Professor Natasa Petronijevic, MD, PhD, has finished her PhD thesis in 2001 at the School of Medicine, University of Belgrade, Serbia. She is a specialist of clinical biochemistry and laboratory medicine. She is a project leader of scientific projects financed by Serbian Government Ministry of Science and a reviewer in several respectable international journals. She is a course director of medical biochemistry and director of PhD studies of neuroscience at the School of Medicine, University of Belgrade. She is a president of  Section for Clinical biochemistry, Serbian Medical Society. She has pulished more than 45 papers in reputed journals.

Abstract:

Decreased bone mineral density, inflammation and dysregulation of hypothalamic–pituitary–adrenal (HPA) axis are described in schizophrenia (SCH). Long-term treatment with antipsychotics is often required for disease control. Perinatal phencyclidine (PCP) administration to rodents represents an animal model of SCH. The aim of this study was to assess the effects of chronic haloperidol and clozapine treatment on bone mass, concentrations of IL-6, TNF-α and corticosterone in serum and the expression of the glucocorticoid receptor (GR), phosphorilated GR (pGR), chaperone and co-chaperone proteins (HSP70, HSP90, FKBP51) and glucocorticoid metabolising enzyme (11β-HSD1) in the brain of adult rats perinatally treated with PCP. Male Wistar  rats were subcutaneously treated on 2nd, 6th, 9th and 12th postnatal day (PN), with either PCP or saline. Antipsychotics were apllied from PN35. Dual X-ray absorptiometry measurements were performed on PN98. Animals were sacrificed on PN100. Concentrations of corticosterone, TNF-α and IL-6 were measured in the serum using ELISA kits. Expressions of proteins were assessed by Western blot. Perinatal PCP administration caused a significant decrease in bone mass and deterioration in bone quality. It didn’t have influence on the interleukine and corticosterone consentrations but changed the expression of GR related proteins indicating increased sensitivity of GR signaling system. Haloperidol had deleterious, while clozapine had protective effect on bones. Both haloperidol and closapine caused decrease of the sensitivity of GR signaling system but clozapine caused significant increase of corticosterone and TNF-α concentrations. Taken together our results indicate that haloperidol affects bones while clozapine alters HPA axes and inflammatory markers.

 

Speaker
Biography:

Naveen Y P is working in Department of Studies in Food Science and Nutrition, University of Mysore, Manasagangothri, Mysore, India.

 

Abstract:

Swietenia mahagoni is a medicinal plant used in various medicinal practices for the treatment of various human ailments. Present study evaluates the anti-diabetic potential of Swietenia mahagoni aqueous extract (MAE) in fructose induced diabetic rats with insulin resistance. Diabetes was induced in the rats by treating the rats with 20 % fructose (W/V) in drinking water for 40 days. Diabetes and hyperinsulinemic condition was confirmed by assessing the glucose and insulin levels in the blood of the experimental rats. After the confirmation of diabetic condition, rats were treated with the MAE and the standard drug metformin and one group left without any treatment and labelled as positive control. MAE treatment improved glucose levels, reduced Insulin levels, improved insulin sensitivity, improved glucose tolerance, improved pancreatic β-cell health, decreased glycated hemoglobin content, reduced lipid peroxides, improved glycogen content and activity of enzymes involved in the synthesis of glycogen in the liver. There was also overall improvement in anti-oxidant status (improved levels of vitamin C, SOD and Catalase and decreased amount of lipid peroxides) of the treated groups when compared with the diabetic control. The treatment also improved the lipid profile (improved HDL and lowered LDL and triglycerides) of diabetic rats. Results confirmed the anti-diabetic potential of MAE in animal model indicating its scope for use as diabetic adjuvant in T-2 diabetic subjects.

Speaker
Biography:

Luiz Augusto da Silva has his expertise in exercise effects in diabetes factors. Sua principal linha de pesquisa é a análise do comportamento de substratos energéticos relacionados a efeitos da cafeína e do exercício físico, buscando alternativas coadjuvantes no tratamento do diabetes mellitus. Novas teorias foram implementadas em seus estudos, como respostas hormonais frente ao exercício físico para o quadro diabético.

Abstract:

Swietenia mahagoni is a medicinal plant used in various medicinal practices for the treatment of various human ailments. Present study evaluates the anti-diabetic potential of Swietenia mahagoni aqueous extract (MAE) in fructose induced diabetic rats with insulin resistance. Diabetes was induced in the rats by treating the rats with 20 % fructose (W/V) in drinking water for 40 days. Diabetes and hyperinsulinemic condition was confirmed by assessing the glucose and insulin levels in the blood of the experimental rats. After the confirmation of diabetic condition, rats were treated with the MAE and the standard drug metformin and one group left without any treatment and labelled as positive control. MAE treatment improved glucose levels, reduced Insulin levels, improved insulin sensitivity, improved glucose tolerance, improved pancreatic β-cell health, decreased glycated hemoglobin content, reduced lipid peroxides, improved glycogen content and activity of enzymes involved in the synthesis of glycogen in the liver. There was also overall improvement in anti-oxidant status (improved levels of vitamin C, SOD and catalase and decreased amount of lipid peroxides) of the treated groups when compared with the diabetic control. The treatment also improved the lipid profile (improved HDL and lowered LDL and triglycerides) of diabetic rats. Results confirmed the anti-diabetic potential of MAE in animal model indicating its scope for use as diabetic adjuvant in T-2 diabetic subjects.

Speaker
Biography:

He is currently an assistant lecturer at Federal University Ndufu-Alike Ikwor,Ebonyi State,Nigeria.His bachelors degree is in Medicine and Surgery(MBBS).Currently he is running a masters degree in Pharmacology and Therapeutics in Ebonyi State University, Nigeria.

Abstract:

Therapeutic drug Monitoring is used to monitor a patient’s progress in the course of treatment and to enhance their therapeutic outcome of patients on Highly Active Antiretroviral Therapy(HAART). It is aimed at identifying drug dependent toxicity in other to adjust dose or change regime among these group of patient. This is extremely important  for drugs with narrow therapeutic index. Chromatography and Immunoassay can be employed in this process especially as because of its specificity and cost effectiveness in estimation of therapeutic drug monitoring. Although this requires expertise and highly trained personnel for this to be carried out. This is most crucial aspect of therapeutic drug monitoring. The setting up of therapeutic drug monitoring is capital intensive both in terms of equipment and personnel. Nigeria been a developing country suffers is its own set back as it requires political will to enable her carry out effective Therapeutic drug monitoring. Therapeutic drug monitoring team comprises of Medical officer,Pharmacologist,PharmarcistandNurse.Pharmacogentics,clinical toxicologist.Trainining and retraining is highly advocated for Doctors in clinical practice as clinical evidence on its own is not enough to determine toxicity. Regretably in most African Countries, this is not done hence the need for advocacy which  is the essence of this paper especially with regard to therapeutic drug monitoring of HAART among HIV patients who will be on life long treatment.

 

Speaker
Biography:

Sunil Gowda S N is pursuing Ph D in Sastra University, India.

Abstract:

Radiotherapy is used to treat tumors of different origins and nature. Lung cancer patients significantly depend on radiotherapy for treatment but often lead to development of radioresistance and increase metastasis of cells. It is interesting that radiation induces the epithelial-mesenchymal transition (EMT), a process by which epithelial cells changes to mesenchymal property and gains enhanced tumor progression capability. Our study investigated the effect of Trichostatin A (TSA), a natural derivate isolated from Streptomyces, upon radiation-induced lung EMT and we tried to understand the role of signaling molecules in irradiated lung cancer cells A549. The cancer cells were irradiated at 8Gy of X-ray using LINAC. The cells were divided into four treatment group untreated control (C), Radiation alone (R), Radiation combined with TSA (R+T) and TSA 100nM (T). Radiation enhanced the migration of cancer cells whereas TSA treatment reduced the migration of cancer cells.  Radiation-induced lung EMT in A549 cells were evidenced by decreased expression of epithelial marker like E-cadherin, Zona occludin and increased expression of N-cadherin and vimentin. The Snail protein, a master regulator of EMT, was observed to be elevated after radiation treatment. In addition, TGF-ß1 signaling (smad2, 3, and 4) proteins were activated upon irradiation. Western blot data were supported by the altered m-RNA expression of E-cadherin. TGF-ß and Snail genes and this effect were reversed by TSA treatment. In addition to this as supportive evidence, we performed docking studies between snail protein and TSA using auto docking software and results suggested that less binding energy is needed for the putative binding of TSA on C-terminal domain of Snail protein. Based on our report, we suggest that TSA can effectively inhibit radiation- induced EMT (i) by altering epithelial and mesenchymal markers (ii) by modulating signaling molecules of TGFß1 pathway (iii) by inhibiting cancer cell migratory potential in A549 cells (iv) by effectively binding to Snail which is an enhancer of EMT. 

 

Speaker
Biography:

He successfully completed  M. Pharm at the age of 25 years from Noakhali Science and Technology University with first position. He also completed  bachelor of pharmacy degree from this same institution with first position too. Presently he is working as a lecturer in pharmacy department of Atish Dipankar University of Science and Technology. He published more than 15 papers in reputed journals and have been serving as an editorial board member of American Journal of plant science.

Abstract:

 
Seed of Vigna unguiculata is commonly used as culinary in Bangladesh and many parts of the world. Besides this, seed of Vigna unguiculata possesses some medicinal values like antioxidant. In this study, seeds of Vigna unguiculata was used to determine anti-obesity and lipid lowering activity. Phytochemicals presents in the seed were evaluated. Total phenolic content (TPC) was determined using folin-ciocalteu method. High fat diet induced obese mice were used for anti-obesity and antihyperlipidemic test. Body weight gain, total cholesterol, triglyceride level were analyzed. Preliminary phytochemical screening showed that amino acid, phenol, alkaloid and terpenes were very strongly present in cowpea seed, while carbohydrate, cardiac glycoside was moderately present. V. unguiculata seed showed significant total phenolic content with a value of 119.716mg of GAE/gm equivalent. Cowpea seed with a dose of 400mg/kg body-weight, showed significant reduction in body weight gain when treated with fat diet compared with normal diet. Cowpea also capable of lowering the serum cholesterol (p<0.01) and serum triglyceride level (p<0.01) more significantly in high fat diet induced obesity. V. unguiculata seeds have total phenolic content significantly and other phytochemicals moderately. It has anti-obesity activity that can interfere with serum cholesterol and triglyceride metabolism. So, it seems like a healthy diet for the management of obesity and other heart related disease.

Speaker
Biography:

Ibrahim Malami has obtained his PhD from Universiti Putra Malaysia. He is a full time Faculty member and a Researcher at the Faculty of Pharmaceutical Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria. His research interest emphasis on cancer research (of colorectal and breast) with focus on chemoprevention, biotherapeutics, and molecular biochemistry. He has published a quite number of papers in high impact journals of peer review process.

Abstract:

Uridine-cytidine kinase 2 is an enzyme that is overexpressed in abnormal cell growth and therefore, implicated in all types of cancers. Due to the selective expression of UCK2 in cancer cells, a selective inhibition of this key enzyme necessitates the discovery of its potential inhibitors for cancer chemotherapy. The present study was carried out to demonstrate the potentials of natural phytochemicals in inhibiting UCK2 enzyme activity useful for colorectal cancer therapy. Flavokawain B and alpinetin were isolated from the the rhizome of Alpinia mutica. These compounds were found to inhibit 50% cell proliferation at low IC50 concentrations. Both compounds were found to reduce ADP production, possibly by inhibiting UCK2 activity using in vitro kinase assay. UCK2 was substantially downregulated in treated HT-29 cells both at gene and protein level. Subsequently, downregulation of 18S ribosomal RNA biosynthesis was confirmed using live cell fluorescence imaging. This study further suggested the possibility of p53 activation through MDM2 downregulation mediated by UCK2 enzyme inhibition. The expression of p53 protein induced p53 transactivation of targets genes that are essential in triggering cell cycle arrest and apoptosis of HT-29 cells. In this respect, apoptosis induction was further confirmed using DNA fragmentation analysis. Results from the in vitro studies have shown the ability of flavokawain B and alpinetin to target UCK2 enzyme specifically, inducing cell cycle arrest and subsequently leading to cancer cell death, possibly through interfering the MDM2-p53 signaling pathway. These phenomena have proven that the bioactive compounds could be useful for future therapeutic use in colorectal cancer.

 

Speaker
Biography:

He completed Ph.D in microbiology. He published 8 research articles in high repute journals and also have 4.4 year experience as lab instructor in the department of bioscience, Integral University, Lucknow. His doctoral work has focused on heavy metal and multidrug resistant gram negative bacteria including E.coli, Enterobactor aerogenes, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhi, Serratia marcescens, Citrobacter amalonaticus and Proteus vulgaris from hospital wastewaterDuring his PhD tenure, apart from the main PhD theme, he also worked on some other areas which are significantly appealing to the global scientific community as they are fascinating to him.

 

Abstract:

The heavy metals are defined as any metallic chemical element that has a comparatively high density at low concentration; it is toxic or poisonous to all forms of life including bacteria. Hospital wastewater discloses the presence of molecules chlorinated in higher concentrations and in a punctual way the presence of heavy metals. Various studies have suggested that hospital wastewater receive inputs of heavy metals from the higher use of radionuclides, pharmaceuticals and antimicrobial solvents. Although some of these heavy metals are essential plant micronutrients and are required or are advantageous for plants and microbial development and growth (Zn, Cu, Fe, Mn, Ni, Mo, Co). The elevated amount and long-term presence of heavy metals, in environment are generally matter of due concern to society as they may adversely affect the quality of soil, water and concerned microbial population. Gram negative bacteria under heavy-metal stress are commonly resistant to their ions and the resistances can often be transferred by conjugation. The higher concentrations of heavy-metal ions form non specific complex compounds in the cell, which ultimately leads to toxic effects. Some heavy-metal cations, e.g. Hg2+ , Cd2+ and Ag2+, form strong toxic complexes, which makes them too dangerous for any physiological function. Therefore, the aim of this study is to reveal the facts as well as positive and negative interaction with gram negative bacteria and indicating the risk of environmental pollution and spread of heavy metal tolerance which may promote the development of resistance to drug among the pathogens.