Hesham N. Mustafa
King Abdulaziz University, Saudi Arabia
Title: Ameliorative potentials of Dexmedetomidine and Benincasa Cerifera extract in renal ischemia/reperfusion injury in streptozotocin induced diabetic model
Biography
Biography: Hesham N. Mustafa
Abstract
Background. Renal ischemia-reperfusion injury (IRI) represents the main reason for acute kidney injury (AKI). Dexmedetomidine (Dex) and Benincasa cerifera (BC) have wide beneficial effects being anti-inflammatory and antioxidants. This study aims illustrate the protective effects of BC and Dex on renal IRI of diabetic model.
Methods. Sixty Male Albino Rats (Wistar strain), weighing 250-300 g. Rats were divided in to 4 groups; Sham group: Non-diabetic. DM+IRI group: STZ-diabetic rats exposed for renal IRI on days 30 after diagnosis of diabetes. DM+IRI+BC group: STZ-diabetic rats were treated with Benincasa Cerfera (500 mg/kg) for 30 days after diagnosis of diabetes then they exposed for renal IRI. DM+IRI+Dex group: STZ-diabetic rats were treated with Dex (100µg/kg intra-peritoneal) 5 minutes before induction of ischemia on day 30 after diagnosis of diabetes then they exposed for renal IRI. Biochemical parameters, histopathological examination and immunohistochemical markers were evaluated.
Results. Significant improvement of biochemical, histopathological and immunohistochemical parameters in (DM+IRI+BC) group while (DM+IRI+Dex) group showed an improving in renal IRI and dyslipidemia.
Conclusion. The present study demonstrated that oxidative stress plays a chief role in renal IRI in STZ-induced diabetic model. Treatment with BC achieved excellent ameliorative effects while treatment with DEX improves renal IRI.