33^rd World Congress on Pharmacology August 26-27, 2020 Barcelona, Spain

Biography:

Aleksandra Kovacevic has completed her PhD in clinical and experimental pharmacology in 2016. She is a pharmacist, specialist for pharmaceutical technology and pharmaco economy. She is an assistant professor of pharmacology and clinical pharmacology at the Military Medical Academy Medical Faculty, University of Defense, Belgrade. She has published more than 35 papers in reputed journals and has been serving as an editorial board member of repute. Viktorija Dragojevic- Simic has completed her PhD in experimental pharmacology and toxicology in 2001. She MD, clinical pharmacologist. She is a full professor in pharmacology and clinical pharmacology at the Military Medical Academy Medical Faculty, University of Defense, Belgrade. She has published more than 100 papers in reputed journals and has been serving as an editorial board member of repute.

Abstract:

Patients with metastatic colorectal cancer (mCRC) have typically overall survival (OS) of approximately 30 months, if multi disciplinary team approach was applied. The first-line treatment comprises cytotoxic agents, a fluoropyrimidines in various protocols, combined with irinotecan or oxaliplatin. Additional benefit, in the terms of clinical outcome for such patients, is shown by adding the monoclonal antibodies (bevacizumab, as anti-VEGF and cetuximab and panitumumab as anti-EGFR). Second-line treatment comprehends adding the anti-angiogenic fusion protein aflibercept or anti-VEGFR2 antibody ramucirumab to the first line protocols. The third line treatment is multi-targeted kinase inhibitor regorafenib. In Serbia, all cytotoxic drugs and monoclonal antibodies bevacizumab and cetuximab, are reimbursed for mCRC patients. Aflibercept, ramucirumab and regorafenib are not on the National Health Insurance Fund (NHIF) reimbursement list. Therefore, we conducted a retrospective randomized case series study, in the large tertiary health care hospital in Serbia. It was concluded that patients with added reimbursed monoclonal antibodies, had 6-month longer OS in five-year period, associated with significantly higher direct medical costs and ICER that was three-fold higher than informal willingness to pay threshold of Serbia. Costs could be significantly decreased only when bevacizumab biosimilars would be available on the Serbian market, but not prior than in 2022, when European Avastin patent expires. European patent on Erbitux expired in 2014; there aren’t any biosimilar competitors in Europe approaching the horizon. Aflibercept is the only third-line treatment option that is registered but not reimbursed in Serbia, and ramucirumab and regorafenib are not registered. As a conclusion, it could be said that novel third-line biological treatment is neither available nor reimbursed for the Serbian patients with mCRC. New patent expiration of the monoclonal antibodies is expecting to allow biosimilar market entry and generate significant savings to the NHIF, which is expected to increase the affordability for mCRC treatment.

Biography:

Hafsa iqbal has completed her pharm-D (Doctor of pharmacy) at the age of 23 years from university f agriculture Faisalabad. Now she is student of m.phill pharmacology in university of agriculture Fisalabad, Pakistan.

Abstract:

Diabetes is a metabolic disorder having serious consequence on health. The pharmacological treatment of diabetes mellitus may involve the use of medications as well as proper diet. Chalcones are flavonoid precursors and have shown a number of antioxidant properties. The current study was conducted to evaluate the antidiabetic and antioxidant activity of newly synthesized chalcone derivative cb6 (E)-3-(4-flourophenyl)-1-phenylprop-2-en-1-one. Hyperglycemia was induced by using alloxan monohydrate intraperitoneally (150mg/kg).In this study 50 albino wister rats were divided into 5 groups. First group was on normal routine diet; second group was given alloxan monohydtrate;  group 3 was given glibenclamide; group 4 ( treated 1) was treated by using research compound at dose rate of 5ml/kg; group 5 ( treated 2) was given research compound at dose rate of 10ml/kg. Antidiabetic activity of chemical compound was determined by performing biochemical and istopathological analysis. Antidiabetic activity of chemical compound in comparison to the activty of synthetic drug glibenclamide was determined. The statistical analysis of variance (ANOA) was performed and the significance among different groups was determined by DMR ( Dunca Multiple Range) test. Results of study indicated that chalcone cb6 significantly reversed the alloxaninduced hyperglycemia by improving biochemical and oxidative stress parameters. Histopatological analysis also showed the antioxidant and antihyperglycemic potential of chalcone cb6. cb6 iproved the activity of endocrine tissues of pancreas by decreasing the oxidative stress by its free radcal scavenging activity

Biography:

Dr. I.A.  Oyemitan obtained B.Pharm., M.Sc., M.Phil., and  PhD (Pharmacology) from Obafemi Awolowo  University, Ile-Ife, Nigeria between 1991 and 2011;  currently a  member  Faculty  of  Pharmacy of the same  university  where  he  teaches  Pharmacology  to  undergraduates  and  postgraduates.  He  did  a  Postdoctoral Fellowship  at  Department  of  Chemical  &  Physical  Sciences,  Walter  Sisulu  University,  Mthatha,  South Africa between 2014 and 2016  and is presently a research  collaborator  with top scientists in the university. His areas of research interests include neuropharmacological and toxicological evaluation of medicinal and aromatic plants. He is presently an Associate Professor of Pharmacology and has over 30 publications

Abstract:

Background: Plectranthus   aegyptiacus is an ethnomedicinal plant found in South-west Nigeria where it is used to manage fever, sensory diseases and cough among other ailments. The objective of this study was to evaluate the neuropharmacological activities of the essential oil of P. aegyptiacus fresh leaf in mice in addition to determine the oil’s chemical composition. Method: Essential oil of P. aegyptiacus (EOPA) was extracted from fresh leaves of the plant by hydro-distillation and analyzed to determine its chemical composition.  The LD50 of the oil was determined orally and intraperitoneally.  The EOPA (50-200 mg/kg, i.p., n=6) was tested for novelty-induced behavioural (NIBs), anxiolytic, sedative, anticonvulsant and analgesic activities using standard protocols. The probable mechanism(s) of the effect of the EOPA on the various neural pathways were studied using various antagonists. Results obtained for the oil were statistically analyzed and compared to negative and positive controls. Results: The  LD50  values  obtained for  the  EOPA were  2154  and 490  mg/kg  for  the  oral  and intraperitoneal routes respectively. The EOPA significantly (p<0.05–0.01) inhibited   all   behavioural   display, significantly (p<0.05-0.01) increased the time spent on the open arms of the elevated plus maze, blocked the hind limb tonic extension on the maximal electric shock and protected the mice against   PTZ–induced mortality, significantly (p<0.05-0.001) shortened sleep latency and prolonged total sleeping time induced by ketamine (100 mg/kg), significantly (p<0.05)) reduced writhings caused by acetic acid (1% v/v) and significantly (p<0.05) increased the reaction time on the hot plate. Flumazenil (2 mg/kg) significantly (p<0.05) reversed the effect of the oil on NIBs; atropine, naloxone and cyproheptadine significantly (p<0.01-0.001) potentiated the inhibitory effect of the oil,   while   yohimbine did   not alter the effect of the oil   on   NIBs.   Major compounds identified in the oil were antioxine, germacrene-D and p-cimene.

Conclusion: The major effect of the oil was depression of the CNS and it demonstrated significant anxiolytic, sedative, anticonvulsant and analgesic activities in mice.  The mechanism   of   action   of   the   oil is   suggested   to   be   mainly   augmentation   of GABAergic   neurotransmission. Furthermore, this research inferentially validated the pharmacological basis for the folkloric use of the plant  

Keywords: Plectranthus aegyptiacus, chemical composition, central nervous system activities, antioxine.

Biography:

Jelalu Kemal Birmeka has completed his BSc at the age of 26 years from Addis Ababa University College of Veterinary Medicine and MSc studies from Haramaya University School of Biological Science and Biotechnology. He is an assistant professor at College of Veterinary Medicine, Haramaya University. He has published 30 papers in reputed journals and has been serving as Coordinator of Cooperative Learning and Quality Assurance of the college.

Abstract:

A study was carried out to evaluate the acaricidal activities of crude methanolic extract of leaves of six medicinal plants: Vernonia amygdalina, Calpurnia aurea, Schinus molle, Ricinus communis, Croto6n macrostachys and Nicotiana tobaccum against Rhipicephalus  decoloratus and Rhipicephalus pulchellus using in-vitro adult immersion test. Five graded concentrations of the crude extracts; 6.25, 12.5, 25, 50 and 100mg/ml were tested and a change in tick viability was recorded for 24 hours. Diazinon (0.1%) and distilled water were used as positive and negative controls, respectively. Phytochemical screening showed the presence of condensed amount of tannins in all extracts. Starting from 30min post exposure the 100mg/ml concentration of C. aurea and R. communis extracts has caused significantly higher mortality. Significant increase in tick mortality was started 2hr post exposure with diazinon and 50 and 100mg/ml concentrations of S. molle. Vernonia amygdalina extract and diazinon showed significant increase in tick mortality 3hr post exposure with 100mg/ml concentration. At 24hr post exposure, diazinon and 50 and 100mg/ml concentrations of all the extracts have caused significantly higher tick mortality than the rest of the concentrations. Higher concentrations of all the extracts had showed a comparable and strong acaricidal effect on Rh decoloratus and Rh. pulchellus having no significant difference with that of positive control (P>0.05) at 24hr post exposure period. Tick killing activity of all evaluated plant extracts increases with increasing exposure time and concentration as well. Thus, all the tested plants could be used against these ticks as potential alternative to substitute commercially available drugs.

Biography:

Kristina Pavlovska is a medical doctor, specialists of Internal medicine. Her present place of employment is the Department of Preclinical and Clinical Pharmacology and Toxicology, Medical faculty, University of Ss. Cyril and Methodius, Skopje, R.of North Macedonia. She holds a MASTERS Degree and PhD Degree in Medical Sciences. Presently she holds faculty position as Associate Professor at the Department of Preclinical and Clinical Pharmacology and Toxicology, Medical faculty, Skopje. Dr Pavlovska has built her experience as investigator/principal investigator in clinical trials and bioequivalence studies. She is author and co-author of more than 20 scientific papers and communications to national and international conferences

Abstract:

We present a case of a 25 year old male patient, with severe active Crohn’s disease, treated with corticosteroids and mesalazine. Due to worsening of the disease, he was referred to the University Clinic of Gastro entero hepatology with anal, scrotal fistula and anal perineum fissure. His condition required total colectomy, terminal ileostomy and excision of  granuloma, fistulotomy and fistula drainage. Postoperatively, he was administered mesalazine, corticosteroids, antibiotics and azathioprine. He did not respond and anti-TNFα was started. During a period of 14 weeks, he received 4 cyclеs of infliximab (5 mg/kg) in combination with azathioprine (100 mg). Despite the combination therapy, he failed to respond and his perianal disease worsened. The patient was a primary non-responder, infliximab was stopped after the end of the 4th cycle. He continued taking azathioprine for 2 years, since no other biologic agent was available. Remission was achieved. This long-term administration resulted in fistulas healing and anal fissure closure. The patient continued being treated with azathioprine for maintaining of the remission with regular laboratory control and close monitoring of possible adverse events. The limited alternative biological therapies, i.e., no possibility to switch to another biologic agent with other mechanisms of action, and no therapeutic concentration monitoring, time to achieve remission for this patient was extended and his quality of life was significantly declined. It is important to point out that in developing countries, biological agents are expensive therapeutic options and are not available in the public health program. This results in poor clinical outcome of the disease.

Biography:

Mahnoor Syed has completed her Pharm-D in 2019. She is recently enrolled in Mphil. pharmacology

Abstract:

Antibiotics support current medicines as their usage has decreased the mortality rate and improved life standards. Though the amount of infections produced by multidrug resistant (MDR) bacteria is growing worldwide and spectrum of terminal infections is becoming certain. Shocking rise in antibiotic resistance of some pathogenic strains of Excherichia coli has developed tangible public health hazards. Some of the genes determines antibiotic resistance amongst the bacteria. QnrA and QnrB are the moxifloxacin resistant genes in Excherichia coli that have shown resistance towards fluoroquinolines via different mechanism. Lactobacillus present in normal microbiota of human intestinal tract and plays a vital role as probiotic. Lactobacillus acts as a pool of gene resistance. Lactobacillus transmits resistant genes to Excherichia coli via horizontal gene transfer method. Clinical samples were collected from human patients and cultured on Eosin methylene blue  agar and sensitivity assay was performed through disc method. Samples were subjected to isloation of gDNA and amplification of bacterial DNA through PCR. Gel electrophoresis was performed for separation and analysis of nucleic acids and gene expression was analyzed thorugh qRT-PCR. The data was subjected to statistical analysis using one-way ANOVA and DMR by using graph pad prism version 6. Results of study have shown that QnrA and QnrB are resistant genes of Excherichia coli against fluoroquinolones and are highly expressed in this specie (P=0.015) compared Lactobacillus (P=0.2). It is concluded that moxifloxacin, a broad spectrum drug is highly resistant to Excherichia coli.

Biography:

Nabeeha Shahab has completed her Pharm-D in 2019. She is recently enrolled in Mphil. pharmacology.

Abstract:

Antimicrobial resistance is an alarming health problem all over the world. Antibiotic resistant bacterial strains are pathogenic and are becoming immune modulator. Studies revelaed that antibiotic-resistant strains are concerned with massive and irrational use of antibiotics. Studies also revealed that increase in resistant bacterial strains have become “nightmare bacteria” so as to “pose a disastrous threat” all over the world. Lactobacilli are the key player in transferring the virulence factors and equally contributing in spreading the resistant genes to other bacterial population through horizontal gene transfer (HGT). In this study we identified the function of lactobacilli in shifting the erm(C) genes to Staphylococcus aureus. The population of the study consist of the patients those who made excessive use of antibiotics. Clinical samples were collected from human patient and cultured on mannitol salt agar medium and sensitivity assay was performed through disc method. Samples were subjected to isolation of g-DNA and amplification of bacterial DNA through PCR followed by Gel electrophoresis and q-RT-PCR for gene expression analysis. erm(C) is the resistant gene against erythromycin and high expression of this gene (P≤0.03) found in Staphylococcus aureus with comparison to lactobacillus. It indicates that erythromycin becomes resistant in Staphylococcus aureus and therapeutic response reduced.

Biography:

Nabil MHAMMED EL BAHIE is a Professor of clinical pharmacology at the Faculty of Medicine Alexandria University, Egypt. He holds MB ChB and MSc (Alexandria) and PhD (Cardiff, UK ) in clinical pharmacology. His main current activity includes teaching clinical pharmacology to under and post graduate

medical students, consultation in therapeutics in addition to research. He used to work as a professor in clinical pharmacology in to Beirut Arab University, Lebanon and The Prince Sattam Bin Abulaziz University, Saudi Arabia; He has published more than 30 papers in reputed journals and supervised 9 Ph.D. and MSc theses.

Abstract:

Abstract

The actions of Vit D are mainly mediated through Vit D receptors (VDR). Different trials studied the possible association between the VDR genetic variants, for e.g. ApaI and TaqI and asthmatic populations in different ethnic groups. During this study, patients were given a daily oral 600 IU of Vit D in addition to their inhaled corticosteroids for 3 months. 64% of the population showed a polymorphism of ApaI and 66.3% of TaqI including both homozygous and heterozygous polymorphisms. Based upon the clinical asthma control level improvement, the patients were categorized as responders (Rs); 62.1% and Non Responders (NRs); 37.9%. The genotypic distribution of both polymorphisms was significantly different between Rs and NRs . Low Vit D mean serum level was detected at the beginning of the study and was significantly increased after 3 months of Vit D supplementation. Pulmonary function tests ( PFTs )results were also significantly ameliorated .While the Rs of different genotypic groups performed significantly better in the PFTs, there was no significant difference between the Vit D serum level between the Rs and the NRs before and after the intervention for the whole population. Daily low dose of Vit D supplementation was beneficial to the asthmatic children as 62.1% of the study population showed a favorable outcome while the role of these specific polymorphisms is not clear yet.

Biography:

Naveed Munir has completed his M.Phil Biochemistry Degree from University of Agriculture, Faisalabad-Pakistan and now He is PhD Biochemistry Scholar at Government College University, Faisalabad-Pakistan. He is the Visiting Lecturer at the same University. He has published more than 35 papers and Book Chapters in reputed journals and Books.

Abstract:

Medicinal herbs and their preparations have been used by the mankind to treat a wide range of disorders since long. Current study was planned to explore the therapeutic potential of ethanolic extract of Epimedium grandiflorum leaves particularly to manage antioxidant and reproductive system disorders in albino male rats. Qualitative and Quantitative analysis explored a wide range of phytoconstituents, and the results of HPLC and FTIR spectroscopy revealed the presence of wide range of phenolic compounds and functional groups, respectively. It was also reported that ethanolic extract exhibited DPPH  scavenging (78.87 ± 5.427 %) and H2O2 scavenging (31.82 ± 3.491 %), antioxidant and reducing power potentials. Further, extract not induced hemolysis (7.56 ± 1.297 %) while have significant clot dissolving (44 ± 5.2 %) potential. In vivo experimentation in albino male rats revealed that administration of plant extract orally for 42 days after intoxication with CCl4 significantly (P<0.05) restore the selected blood parameters including liver enzymes, renal profiles, and stress markers. Moreover, administration of ethanolic extract significantly (P<0.05) restored reproductive hormones including testosterone, luteinizing hormone (LH), follicle stimulating hormone (FSH) and prolactin while significant (P<0.05) decreased levels of progesterone and estradiol toward a normal level in dose dependent manner. On histological examination of testicular tissue revealed significant (P<0.05) improvement in the structural architecture, especially in animals received ethanolic extract in high dose (200 mg/Kg b.w.) as compared to both positive control groups. It could be concluded that E. grandiflorum medicinal plant has significant antioxidant and reproductive hormones restoring capacity. However, more research is required to isolate the novel compounds from this therapeutic plant to address the healthcare problems particularly impotency.

Biography:

Snezana Mugosa has completed his PhD in 2015 at the age of 35 years. In 2011 she passed the Internal Medicine Certification Exam at the Faculty of Medicine of the University of Belgrade. Since 2012 has been working as the the Head of the Department for clinical trials of medicines for human and veterinary use and assessment of preclinical and clinical documentation for marketing authorization in the Agency for Medicines and Medical Devices of Montenegro. Since 2004 she has been engaged at the Faculty of Medicine, Podgorica on the group of pharmacological subjects, initially as an assisstant, and then, since 2015, as a lecturer in Pharmacology II, Pharmacology I and II and Pharmacokinetics. She has published more than 25 papers in reputed journals

Abstract:

Background: This study addresses the genetic and nongenetic factors associated with increased risk of having major adverse cardiac events in Montenegrians treated with clopidogrel.The aim of this study was to provide the analysis of genetic and non-genetic factors that influence clopidogrel efficacy in cardiology patients.

Methods: We have conducted a prospective study in 200 hospitalized patients. CYP2C19 genetic testing was conducted, and the PREDICT score was calculated in 102 out of 200 patients treated with clopidogrel in order to determine the influence of genetic and non-genetic factors on outcomes of interest. Adverse cardiovascular events and adverse reactions to clopidogrel were assessed during 12 months follow up period.

Results: In univariate logistic regression model, statistically significant predictors of the outcome of interest are: the PREDICT score (p <0.001), enzymatic activity [slow metabolizers (p<0.001) compared to the rapid, extensive and intermediate metabolizers as a reference category] and concomitant use of other drugs that are also metabolized through CYP2C19 (p = 0.030). In multivariate logistic regression model, predictors from the model of univariate logistic regression which were statistically significant at the significance level of 0.05 were included. The model contains three predictors - PREDICT score, enzymatic activity and concomitant administration of other drugs that are metabolized via the same CYP enzyme. The whole model was statistically significant (p < 0.001). There is no significant multicollinearity or interaction between the predictors (p = 0.002 and 0.009, respectively).

Conclusions: In assessing the clopidogrel resistance in cardiology patients the stepwise approach could be used, combining the PREDICT score, platelet aggregation test, and genetic testing for CYP2C19 polymorphism.

Biography:

Dr. Shereen Nader Raafat currently working in the department of pharmacology and toxicology (Pharmaceutical Sciences), Faculty of Dentistry, The British University in Egypt (BUE). She has done her graduation from the Faculty of Pharmacy, Ain Shams University. First she had Masters Degree in Clinical Pharmacy from Ain Shams University with the opportunity to implement clinical pharmacy practice where she first worked in The National Institute of Urology and Nephrology, where she aimed to improve general patient’s health and pharmacy practice. Since joining academia, she has researched extensively problems related to the orthopedic field. She has attended various national and international conferences and workshops on experimental animals and tissue culture. Recently she had her PhD Degree in the Pharmacology Department, Cairo University. Recent research has been published in The Journal of Bone which had a powerful impact not only on the field of orthopaedic s but also the field of dentistry. In addition, she is a member in the Egyptian Society of Pharmacology & Experimental Therapeutics Journal.

Abstract:

Aim: This study compares the bone regenerative power of SIM and PRF and the combination added locally on induced bone defect and their effect on inflammatory markers.

Materials and methods: A critical size bone defect was induced in 48 male albino rats of average weight 150–200 g and were divided into 4 groups according to the filling material. Control, PRF, SIM, and SIM/PRF group. Each group was subdivided according to the sacrificing period into two subgroups (one and two-months postoperatively). Tibial specimens were evaluated histologically using Hematoxylin and eosin (H&E) stain, serum inflammatory markers (IL-1β, IL-6, IL-10 and TNF-α) 6 days post-surgery using ELISA technique.

Results: Bone specimens of the SIM group and the combination showed well observed darkly stained areas of matured bone compared to the other groups especially two-month postoperatively, the SIM/PRF group showed matured bone trabeculae surrounding bone marrow spaces which appeared densely stained with normal osteocytes lacunae. Resting and reversal lines were obviously detected in the combination group only, denoting high bone remodeling activity in this group. PRF and SIM decreased significantly the pro-inflammatory IL-1β serum levels compared to the control group, SIM loaded on PRF showed the highest statistical significant decrease in IL-1β serum concentration (P<0.001). IL-6 and TNF-α serum levels didn’t differ significantly between the control and PRF group, but showed statistical significant decrease in the SIM and SIM//PRF groups. In contrast PRF, SIM and SIM/PRF significantly increased IL-10 serum levels compared to the control group with the highest significant increase in the SIM/PRF group (P<0.001).

Conclusion: Comparing SIM with PRF each as a sole filling material in the bone defect, SIM showed more enhanced bone regeneration and more powerful anti-inflammatory effect, while the combination has proven to cause the most significant bone formation but without significant effect on inflammation

Biography:

Dr. Shereen Nader Raafat currently working in the department of pharmacology and toxicology (Pharmaceutical Sciences), Faculty of Dentistry, The British University in Egypt (BUE). She has done her graduation from the Faculty of Pharmacy, Ain Shams University. First she had Masters Degree in Clinical Pharmacy from Ain Shams University with the opportunity to implement clinical pharmacy practice where she first worked in The National Institute of Urology and Nephrology, where she aimed to improve general patient’s health and pharmacy practice. Since joining academia, she has researched extensively problems related to the orthopedic field. She has attended various national and international conferences and workshops on experimental animals and tissue culture. Recently she had her PhD Degree in the Pharmacology Department, Cairo University. Recent research has been published in The Journal of Bone which had a powerful impact not only on the field of orthopaedic s but also the field of dentistry. In addition, she is a member in the Egyptian Society of Pharmacology & Experimental Therapeutics Journal.

Abstract:

Aim: This study compares the bone regenerative power of SIM and PRF and the combination added locally on induced bone defect and their effect on inflammatory markers.

Materials and methods: A critical size bone defect was induced in 48 male albino rats of average weight 150–200 g and were divided into 4 groups according to the filling material. Control, PRF, SIM, and SIM/PRF group. Each group was subdivided according to the sacrificing period into two subgroups (one and two-months postoperatively). Tibial specimens were evaluated histologically using Hematoxylin and eosin (H&E) stain, serum inflammatory markers (IL-1β, IL-6, IL-10 and TNF-α) 6 days post-surgery using ELISA technique.

Results: Bone specimens of the SIM group and the combination showed well observed darkly stained areas of matured bone compared to the other groups especially two-month postoperatively, the SIM/PRF group showed matured bone trabeculae surrounding bone marrow spaces which appeared densely stained with normal osteocytes lacunae. Resting and reversal lines were obviously detected in the combination group only, denoting high bone remodeling activity in this group. PRF and SIM decreased significantly the pro-inflammatory IL-1β serum levels compared to the control group, SIM loaded on PRF showed the highest statistical significant decrease in IL-1β serum concentration (P<0.001). IL-6 and TNF-α serum levels didn’t differ significantly between the control and PRF group, but showed statistical significant decrease in the SIM and SIM//PRF groups. In contrast PRF, SIM and SIM/PRF significantly increased IL-10 serum levels compared to the control group with the highest significant increase in the SIM/PRF group (P<0.001).

Conclusion: Comparing SIM with PRF each as a sole filling material in the bone defect, SIM showed more enhanced bone regeneration and more powerful anti-inflammatory effect, while the combination has proven to cause the most significant bone formation but without significant effect on inflammation

Biography:

Jeremy Everett is the Professor of Pharmaceutical Technologies at the University of Greenwich UK and Visiting Professor in the Department of Surgery and Cancer at Imperial College. Jeremy conducts research in metabonomics/metabolomics and pharmaco-metabonomics. His current work is focused on genotype – metabotype correlations in the areas of obesity and ageing and he is a co-inventor on a recently filed patent on an anti-obesity agent. Jeremy received both his BSc and PhD in chemistry from Nottingham University, UK. He did post-doctoral studies at McMaster University and at McGill University in Canada. Jeremy is a Fellow of the Royal Society of Chemistry and a Chartered Chemist, a Member of the American Chemical Society, a Fellow of the Higher Education Academy and is an author or co-author on over 100 peer-reviewed publications and patents, with over 4,900 citations to date and an h-index of 31. He has delivered over 60 invited lectures.

Abstract:

Metabolic profiling or metabonomics is an important systems biology methodology for disease diagnosis and prognosis. It is also an excellent methodology for the phenotyping of genetically modified mice. We recently used this technology in collaboration with the group of Professor Elizabeth Shephard at UCL, to investigate the metabolic phenotype of a genetically modified  mouse that had an unusual, lean phenotype. The genetic modification in this mouse caused a change in its microbiome, and the production by the microbiome, of a new metabolite X. We identified this compound by spectroscopic methods and subsequently showed it to have antiobesity properties and to be responsible for the lean clinical phenotype. This talk will provide an overview of this work and the properties of compound X.

Biography:

Dr. Ishita Sharma is a practising Obstetrician & Gynecologist with an experience of 17 years. She is located in Tarn Taran. Dr. Ishita Sharma practices at the Guru Nanak Dev Super Specialty Hospital in Tarn Taran. The Guru Nanak Dev Super Specialty Hospital is situated at Guru Nanak Dev Super Speciality Hospital, Goindwal Sahib Road Bachre, Tarn Taran. She pursued her MBBS in the year 2003 from Calcutta University, West Bengal. Dr. Ishita Sharma is an experienced, skilled and awarded doctor in her field of specialization. She worked at Guru Nanak Dev Super Specialty Hospital from 2017 to 2019.

Abstract:

Aims: This study was conducted with the aim of comparing the efficacy of oral misoprostol 600 mcg with intramuscular oxytocin 10 IU in the active management of third stage of labour.

Methods:

This prospective comparative study was performed at Department of Obstetrics and Gynaecology, South Eastern Railways, Kolkata, a tertiary care hospital offering referral services to pregnant women.

The aimed at to compare the efficacy of oral misoprostol with intramuscular oxytocin in the third stage of labour to prevent of postpartum hemorrhage. 60 women without risk of PPH were randomly allocated to receive either 600 mcg misoprostol orally (Group I) or 10 unit of oxytocin intramuscularly (Group II) within 1 minute of delivery. The efficacy and the safety of these two drugs were analyzed on the basis of percentages fall in hemoglobin (Hb) and hematocrit (Hct) level from before delivery to 8 completed hours after delivery, need for additional uterotonic agents, need for exploration and uterine evacuation, need for blood transfusion, duration of third stage of labour and the numbers of retained placenta and need for MRP.

Results: Oral misoprostol was observed to be equally effective as intramuscular oxytocin in prevention of post-partum hemorrhage (PPH). There was no statistical difference in the duration of third stage of labour, need for additional uterotonics, need for uterine exploration/evacuation and need for blood transfusion in the two groups.

Conclusions: Routine use of oral misoprostol 600 mcg appears to be as effective as 10 IU intramuscular oxytocin in minimizing blood loss during the third stage of labour.

Keywords: misoprostol; active management of third stage of labour; side effects; oxytocin; blood loss; PPH

Biography:

Assistant Professor, Former Researcher CSIR-CDRI, Expert in Clinical Research, Pharmacovigilance, Medical Writing, Medical Coding, Medical Transcription, Computer Added Drug Design, Molecular and Structural Biology, Bio Informatics,3D Printing Technology.

Abstract:

The whole world is in the grip of the COVID-19 (corona virus) Pandemic. The spread of the virus has had unprecedented consequences on the world economy. In this context of deteriorating economic situation due to corona virus, Indian industries have to bear huge financial burden and serious consequences. The COVID-19 pandemic affected the global economy in various sectors. It has come at a cost such as the collapse of health systems, the tragic financial crisis or the recession. Therefore, this article is intended to study the impact of the corona virus on various sectors of the Indian economy, to highlight the reasons for India's interest in the post - epidemic period and to explain important business survival strategies to overcome these difficulties.

Keywords: COVID-19, corona virus, social policy, financial crisis, monetary policy, central economic impact

Biography:

Mr. Prashant Gupta is working Assistant professor in department of Pharmacology, at Shree S. K. Patel College of Pharmaceutical Education & Research, GANPAT University, Mehsana Gujarat. He has submitted his PhD thesis from the department of Pharmacology and Toxicology. His role as a PhD research scholar at NIPER Mohali includes drug combination screening, handling of tuberculosis bacteria, primary cell line culture, working with endogenous opioids and peptide screening. His academic credentials include a B. Pharm from GGU (central university), Bilaspur; (2012), M. S. (Pharm.) in Regulatory Toxicology from NIPER, Mohali (2014). Previously, He has worked at Sun Pharmaceutical Industries Limited (Drug Safety Evaluation department, GLP), Gurugram for 14 months. At SUN Pharma, he involved in various toxicities studies (Acute, Repeated dose, 4 days, 14 days, 28 days), which includes animal handling, dosing, sample collection from study animals, blood withdrawal, necropsy, organ collection and weighing, data collection and report preparation. He is good at GraphPad Prism, ImageJ, TOPKAT, DEREK software’s and well versed with MS-Office (Word, Excel, PowerPoint), which are very much required for research work. He has well skilled in retrieving data from databases like PubMed, ScienceDirect and Google Scholar etc. He had considerable theoretical exposure to Anatomy & Physiology in Health and Disease, Clinical Pharmacology, Biotechnology, Pharm. Analysis etc. during his M.S. (Pharm.) and acquainted with the guidelines of OECD and ICH.He has and more than 6 years’ experience in research & teaching (including Ph.D. tenure) in the Pharmacology & Toxicology area at different organizations.

Abstract:

Background: Tuberculosis (TB) is one of the world’s deadliest diseases as one third of the world’s population is infected with TB. The WHO TB statistics for India for 2017 give an estimated incidence figure of 2.8 million cases of TB. Bioimmunotherapy approach along with the conventional drug therapy can be a new approach to tackle the problems associated with TB treatment. rmGM-CSF can exert its bioimmunotherapeutic effect through three possible mechanisms viz. activation of effector functions of macrophages, regulation of cytokine network and nitric oxide production. m-ENK has been reported to modulate nitrite and TNF-α production, and effector functions of macrophages. Therefore, we postulate that rmGM-CSF and M-ENK co- treatment imparts protection against TB. Materials &amp; Methods: Here, we have tested the M-ENK and rmGM-CSF combination at different concentration in mouse peritoneal macrophages infected with Mycobacterium tuberculosis (H37Ra) and calculate the % phagocytic by ZN staining and colony forming units Results: The different concentration of M-ENK (10 -7 , 10 -9 and 10 -11 M) and rmGM-CSF (50 Results: The different concentration of M-ENK (10 -7 , 10 -9 and 10 -11 M) and rmGM-CSF (50 pg/ml) were tested in mouse peritoneal macrophages in vitro and observed the phagocytosis process in experiments. The combined effect of M-ENK and rmGM-CSF at 50 pg/ml and 10 -9 M dose showed maximum phagocytic activity (70-80 %) using intra-macrophage phagocytic assay  on murine macrophages, in vitro. The reduction in colony forming units (cfu) was also observed in combined dose combination of the M-ENK and rmGM-CSF at 50 pg/ml and 10 -9 M dose treatment. Conclusion: We conclude that Bioimmunotherapy with rmGM-CSF and M-ENKs co-treatment can be a possible alternative to control tuberculosis infection.

Keyword: Bioimmunotherapy, rmGM-CSF and M-ENK (methionine-Enkephalin)