Aline Diogo Marinho
Federal University of Ceara, Brazil
Title: Study of renal and cytotoxic effects induced by Bothrops pauloensis venom
Biography
Biography: Aline Diogo Marinho
Abstract
Acute renal failure (ARF) is one of the most serious complications of Bothrops snake bites. Pathogenesis of ARF in snakebite envenomation may involve hemodynamic disturbances, immunologic reactions and direct nephrotoxicity, however, its pathogenesis remains obscure. Bothrops pauloensis is found in the State of São Paulo, Southeast region of Brazil. The aim of the work was to study Bothropos pauloensis venom (BpV) in rat isolated perfused kidneysand cytotoxicity on renal tubular cells Mardin-Darby Canine Kidney (MDCK). The renal effects were compared to a control group perfused with modified Krebs–Henseleit solution alone. BpV decreased the perfusion pressure (PP), renal vascular resistance (RVR),urinary flow (UF), glomerular filtration rate (GFR) and the percent sodium, potassium and chloride tubular transport (%TNa+, %TK+, %TCl−). The treatment with BpV caused decrease in cell viability to the lowest concentration tested with an IC (50) of 7.5 μg/mL. Flow cytometry with annexin V and propidium iodide showed that cell death, suggesting the participation of apoptosis and late apoptosis on BpV-induced cell death. Through caspases 3 and 7 activation, mitochondrial membrane potential collapse and ROS overproduction. These ï¬ndings demonstrated that BpV cytotoxicity on renal epithelial cells might be responsible for the nephrotoxicity observed in isolated kidney. The characterization of the effects in the isolated kidney and renaltubular cells gives strong evidences that the acute renal failure induced by this venom is a result of the direct nephrotoxicity which may involve the cell death mechanism.